Whole Genome Sequencing demonstrates that geographic variation of Escherichia coli O157 genotypes dominates host association

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dc.citation.volume5
dc.contributor.authorStrachan NJC
dc.contributor.authorRotariu O
dc.contributor.authorLopes B
dc.contributor.authorMacrae M
dc.contributor.authorFairley S
dc.contributor.authorLaing C
dc.contributor.authorGannon V
dc.contributor.authorAllison LJ
dc.contributor.authorHanson MF
dc.contributor.authorDallman T
dc.contributor.authorAshton P
dc.contributor.authorFranz E
dc.contributor.authorVan Hoek AHAM
dc.contributor.authorFrench NP
dc.contributor.authorGeorge T
dc.contributor.authorBiggs PJ
dc.contributor.authorForbes KJ
dc.date.available2015-10-07
dc.date.available2015-08-19
dc.date.issued2015-10-07
dc.description.abstractGenetic variation in an infectious disease pathogen can be driven by ecological niche dissimilarities arising from different host species and different geographical locations. Whole genome sequencing was used to compare E. coli O157 isolates from host reservoirs (cattle and sheep) from Scotland and to compare genetic variation of isolates (human, animal, environmental/food) obtained from Scotland, New Zealand, Netherlands, Canada and the USA. Nei's genetic distance calculated from core genome single nucleotide polymorphisms (SNPs) demonstrated that the animal isolates were from the same population. Investigation of the Shiga toxin bacteriophage and their insertion sites (SBI typing) revealed that cattle and sheep isolates had statistically indistinguishable rarefaction profiles, diversity and genotypes. In contrast, isolates from different countries exhibited significant differences in Nei's genetic distance and SBI typing. Hence, after successful international transmission, which has occurred on multiple occasions, local genetic variation occurs, resulting in a global patchwork of continental and trans-continental phylogeographic clades. These findings are important for three reasons: first, understanding transmission and evolution of infectious diseases associated with multiple host reservoirs and multi-geographic locations; second, highlighting the relevance of the sheep reservoir when considering farm based interventions; and third, improving our understanding of why human disease incidence varies across the world.
dc.description.publication-statusPublished
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000362330700001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=c5bb3b2499afac691c2e3c1a83ef6fef
dc.identifierARTN 14145
dc.identifier.citationSCIENTIFIC REPORTS, 2015, 5
dc.identifier.doi10.1038/srep14145
dc.identifier.elements-id259049
dc.identifier.harvestedMassey_Dark
dc.identifier.issn2045-2322
dc.relation.isPartOfSCIENTIFIC REPORTS
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Com- mons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
dc.titleWhole Genome Sequencing demonstrates that geographic variation of Escherichia coli O157 genotypes dominates host association
dc.typeJournal article
pubs.notesNot known
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Sciences
pubs.organisational-group/Massey University/College of Sciences/School of Natural Sciences
pubs.organisational-group/Massey University/College of Sciences/School of Veterinary Science
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