Quartz crystal microbalance genosensing of brettanomyces bruxellensis yeast in wine using a rapid and efficient drop and collect protocol

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dc.citation.issue5
dc.citation.volume11
dc.contributor.authorPoggesi S
dc.contributor.authorZhou L
dc.contributor.authorBariani GC
dc.contributor.authorMittapalli R
dc.contributor.authorManzano M
dc.contributor.authorIonescu RE
dc.contributor.editorGuo P
dc.contributor.editorPortalone G
dc.contributor.editorPirri F
dc.date.accessioned2023-10-26T00:42:34Z
dc.date.accessioned2023-10-26T20:32:35Z
dc.date.available2021-05-18
dc.date.available2023-10-26T00:42:34Z
dc.date.available2023-10-26T20:32:35Z
dc.date.issued2021-05-18
dc.date.updated2023-10-24T21:51:28Z
dc.description.abstractA miniaturized quartz crystal microbalance (QCM) genosensor is proposed for sensitive and real-time detection of short ssDNA sequences (53 bp) or DNA extracted from Brettanomyces bruxellensis (Brett) yeast cells. The presence of Brett yeast causes a depreciation of the quality of aged fine wines, producing molecules of unpleasant odors and biogenic amines that are harmful to human health. More specifically, standard quartz crystal (S-QCM) and homemade 4 nm gold transmission electron microscopy (TEM)-grid patterned quartz (multi-TEM QCM) are herein proposed for biofunctionalization steps with different ssDNA sequences. By employing a rapid and efficient drop and collect protocol, the specific detection of 1 pg/µL ssDNA Brett of a short sequence and 100 ng/µL DNA of B. bruxellensis extracted from a wine sample (VR2008) is reported.
dc.description.confidentialfalse
dc.edition.editionMay 2021
dc.identifier562
dc.identifier.citationPoggesi S, Zhou L, Bariani GC, Mittapalli R, Manzano M, Ionescu RE. (2021). Quartz crystal microbalance genosensing of brettanomyces bruxellensis yeast in wine using a rapid and efficient drop and collect protocol. Crystals. 11. 5.
dc.identifier.doi10.3390/cryst11050562
dc.identifier.eissn2073-4352
dc.identifier.elements-typejournal-article
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/68972
dc.languageEnglish
dc.publisherMDPI (Basel, Switzerland)
dc.publisher.urihttps://www.mdpi.com/2073-4352/11/5/562
dc.relation.isPartOfCrystals
dc.rights(c) 2021 The Author/sen_US
dc.rightsCC BYen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subjectquartz crystal microbalance
dc.subjectmulti-TEMs patterned crystal
dc.subjectgenosensing
dc.subjectBrettanomyces bruxellensis
dc.subjectdrop and collect protocol
dc.titleQuartz crystal microbalance genosensing of brettanomyces bruxellensis yeast in wine using a rapid and efficient drop and collect protocol
dc.typeJournal article
pubs.elements-id456880
pubs.organisational-groupOther
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