Transcriptomic Identification of a Unique Set of Nodule-Specific Cysteine-Rich Peptides Expressed in the Nitrogen-Fixing Root Nodule of Astragalus sinicus

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dc.citation.issue10
dc.citation.volume35
dc.contributor.authorWei F
dc.contributor.authorLiu Y
dc.contributor.authorZhou D
dc.contributor.authorZhao W
dc.contributor.authorChen Z
dc.contributor.authorChen D
dc.contributor.authorLi Y
dc.contributor.authorZhang X-X
dc.coverage.spatialUnited States
dc.date.accessioned2024-01-18T01:42:13Z
dc.date.accessioned2024-07-25T06:44:42Z
dc.date.available2022-09-27
dc.date.available2024-01-18T01:42:13Z
dc.date.available2024-07-25T06:44:42Z
dc.date.issued2022-10-08
dc.description.abstractLegumes in the inverted repeat-lacking clade (IRLC) each produce a unique set of nodule-specific cysteine-rich (NCR) peptides, which act in concert to determine the terminal differentiation of nitrogen-fixing bacteroid. IRLC legumes differ greatly in their numbers of NCR and sequence diversity. This raises the significant question how bacteroid differentiation is collectively controlled by the specific NCR repertoire of an IRLC legume. Astragalus sinicus is an IRLC legume that forms indeterminate nodules with its microsymbiont Mesorhizobium huakuii 7653R. Here, we performed transcriptome analysis of root and nodule samples at 3, 7, 14, 28 days postinoculation with M. huakuii 7653R and its isogenic ∆bacA mutant. BacA is a broad-specificity peptide transporter required for the host-derived NCRs to target rhizobial cells. A total of 167 NCRs were identified in the RNA transcripts. Comparative sequence and electrochemical analysis revealed that A. sinicus NCRs (AsNCRs) are dominated by a unique cationic group (termed subgroup C), whose mature portion is relatively long (>60 amino acids) and phylogenetically distinct and possessing six highly conserved cysteine residues. Subsequent functional characterization showed that a 7653R variant harboring AsNCR083 (a representative of subgroup C AsNCR) displayed significant growth inhibition in laboratory media and formed ineffective white nodules on A. sinicus with irregular symbiosomes. Finally, bacterial two-hybrid analysis led to the identification of GroEL1 and GroEL3 as the molecular targets of AsNCR067 and AsNCR076. Together, our data contribute to a systematic understanding of the NCR repertoire associated with the A. sinicus and M. huakuii symbiosis.
dc.description.confidentialfalse
dc.edition.editionOctober 2022
dc.format.pagination893-905
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/35762679
dc.identifier.citationWei F, Liu Y, Zhou D, Zhao W, Chen Z, Chen D, Li Y, Zhang X-X. (2022). Transcriptomic Identification of a Unique Set of Nodule-Specific Cysteine-Rich Peptides Expressed in the Nitrogen-Fixing Root Nodule of Astragalus sinicus.. Mol Plant Microbe Interact. 35. 10. (pp. 893-905).
dc.identifier.doi10.1094/MPMI-03-22-0054-R
dc.identifier.eissn1943-7706
dc.identifier.elements-typejournal-article
dc.identifier.issn0894-0282
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/70786
dc.languageeng
dc.publisherThe American Phytopathological Society in cooperation with the International Society for Molecular Plant-Microbe Interactions
dc.publisher.urihttps://apsjournals.apsnet.org/doi/full/10.1094/MPMI-03-22-0054-R
dc.relation.isPartOfMol Plant Microbe Interact
dc.rights(c) 2022 The Author/s
dc.rightsCC BY-NC-ND 4.0
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAstragalus sinicus
dc.subjectIRLC legumes
dc.subjectNCR peptides
dc.subjectRhizobium
dc.subjectbacteroid differentiation
dc.subjectindeterminate nodule
dc.subjectnitrogen fixation
dc.subjectCysteine
dc.subjectFabaceae
dc.subjectNitrogen
dc.subjectNitrogen Fixation
dc.subjectPeptides
dc.subjectRNA
dc.subjectRoot Nodules, Plant
dc.subjectSymbiosis
dc.subjectTranscriptome
dc.titleTranscriptomic Identification of a Unique Set of Nodule-Specific Cysteine-Rich Peptides Expressed in the Nitrogen-Fixing Root Nodule of Astragalus sinicus
dc.typeJournal article
pubs.elements-id454153
pubs.organisational-groupOther
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