Biofilm formation, sodium hypochlorite susceptibility and genetic diversity of Vibrio parahaemolyticus

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dc.citation.volume385
dc.contributor.authorWang D
dc.contributor.authorFletcher GC
dc.contributor.authorOn SLW
dc.contributor.authorPalmer JS
dc.contributor.authorGagic D
dc.contributor.authorFlint SH
dc.coverage.spatialNetherlands
dc.date.accessioned2023-10-24T21:58:01Z
dc.date.accessioned2023-10-25T20:49:30Z
dc.date.available2022-11-08
dc.date.available2023-10-24T21:58:01Z
dc.date.available2023-10-25T20:49:30Z
dc.date.issued2023-01-16
dc.date.updated2023-10-16T21:17:41Z
dc.description.abstractVibrio parahaemolyticus is a marine oriented pathogen; and biofilm formation enables its survival and persistence on seafood processing plant, complicating the hygienic practice. The objectives of this study are to assess the ability of V. parahaemolyticus isolated from seafood related environments to form biofilms, to determine the effective sodium hypochlorite concentrations required to inactivate planktonic and biofilm cells, and to evaluate the genetic diversity required for strong biofilm formation. Among nine isolates, PFR30J09 and PFR34B02 isolates were identified as strong biofilm forming strains, with biofilm cell counts of 7.20, 7.08 log10 CFU/cm2, respectively, on stainless steel coupons after incubation at 25 °C. Free available chlorine of 1176 mg/L and 4704 mg/L was required to eliminate biofilm cells of 1.74-2.28 log10 CFU/cm2 and > 7 log10 CFU/cm2, respectively, whereas 63 mg/L for planktonic cells, indicating the ineffectiveness of sodium hypochlorite in eliminating V. parahaemolyticus biofilm cells at recommended concentration in the food industry. These strong biofilm-forming isolates produced more polysaccharides and were less susceptible to sodium hypochlorite, implying a possible correlation between polysaccharide production and sodium hypochlorite susceptibility. Genetic diversity in mshA, mshC and mshD contributed to the observed variation in biofilm formation between isolates. This study identified strong biofilm-forming V. parahaemolyticus strains of new multilocus sequence typing (MLST) types, showed a relationship between polysaccharide production and sodium hypochlorite resistance.
dc.description.confidentialfalse
dc.format.extent110011-
dc.identifier110011
dc.identifierS0168-1605(22)00483-4
dc.identifierhttps://www.ncbi.nlm.nih.gov/pubmed/36370527
dc.identifier.citationWang D, Fletcher GC, On SLW, Palmer JS, Gagic D, Flint SH. (2023). Biofilm formation, sodium hypochlorite susceptibility and genetic diversity of Vibrio parahaemolyticus.. Int J Food Microbiol. 385. (pp. 110011-).
dc.identifier.doi10.1016/j.ijfoodmicro.2022.110011
dc.identifier.eissn1879-3460
dc.identifier.elements-typejournal-article
dc.identifier.harvestedMassey_Dark
dc.identifier.issn0168-1605
dc.identifier.urihttp://hdl.handle.net/10179/20385
dc.languageeng
dc.publisherElsevier BV
dc.publisher.urihttps://www.sciencedirect.com/science/article/pii/S0168160522004834
dc.relation.isPartOfInt J Food Microbiol
dc.rights(c) 2022 The Author/sen_US
dc.rightsCC BY-NC-ND 4.0en_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.subjectBiofilm
dc.subjectGenomic feature
dc.subjectMultilocus sequence typing (MLST)
dc.subjectSodium hypochlorite
dc.subjectWhole genome sequencing (WGS)
dc.subjectVibrio parahaemolyticus
dc.subjectSodium Hypochlorite
dc.subjectMultilocus Sequence Typing
dc.subjectBiofilms
dc.subjectGenetic Variation
dc.titleBiofilm formation, sodium hypochlorite susceptibility and genetic diversity of Vibrio parahaemolyticus
dc.typeJournal article
pubs.elements-id457974
pubs.organisational-groupOther
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