The Effects of General Anaesthesia and Light on Behavioural Rhythms and GABAA Receptor Subunit Expression in the Mouse SCN.

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dc.citation.issue3
dc.citation.volume3
dc.contributor.authorChong J
dc.contributor.authorCheeseman JF
dc.contributor.authorPawley MDM
dc.contributor.authorKwakowsky A
dc.contributor.authorWarman GR
dc.contributor.editorCajochen C
dc.coverage.spatialSwitzerland
dc.date.accessioned2024-01-07T22:15:41Z
dc.date.accessioned2024-07-25T06:45:01Z
dc.date.available2021-09-17
dc.date.available2024-01-07T22:15:41Z
dc.date.available2024-07-25T06:45:01Z
dc.date.issued2021-09-17
dc.description.abstractGeneral anaesthesia (GA) is known to affect the circadian clock. However, the mechanisms that underlie GA-induced shifting of the clock are less well understood. Activation of γ-aminobutyric acid (GABA)-type A receptors (GABAAR) in the suprachiasmatic nucleus (SCN) can phase shift the clock and thus GABA and its receptors represent a putative pathway via which GA exerts its effect on the clock. Here, we investigated the concurrent effects of the inhalational anaesthetic, isoflurane, and light, on mouse behavioural locomotor rhythms and on α1, β3, and γ2 GABAAR subunit expression in the SCN of the mouse brain. Behavioural phase shifts elicited by exposure of mice to four hours of GA (2% isoflurane) and light (400 lux) (n = 60) were determined by recording running wheel activity rhythms in constant conditions (DD). Full phase response curves for the effects of GA + light on behavioural rhythms show that phase shifts persist in anaesthetized mice exposed to light. Daily variation was detected in all three GABAAR subunits in LD 12:12. The γ2 subunit expression was significantly increased following GA in DD (compared to light alone) at times of large behavioural phase delays. We conclude that the phase shifting effect of light on the mouse clock is not blocked by GA administration, and that γ2 may potentially be involved in the phase shifting effect of GA on the clock. Further analysis of GABAAR subunit expression in the SCN will be necessary to confirm its role.
dc.description.confidentialfalse
dc.edition.editionSeptember 2021
dc.format.pagination482-494
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/34563056
dc.identifier.citationChong J, Cheeseman JF, Pawley MDM, Kwakowsky A, Warman GR. (2021). The Effects of General Anaesthesia and Light on Behavioural Rhythms and GABAA Receptor Subunit Expression in the Mouse SCN.. Clocks Sleep. 3. 3. (pp. 482-494).
dc.identifier.doi10.3390/clockssleep3030034
dc.identifier.eissn2624-5175
dc.identifier.elements-typejournal-article
dc.identifier.issn2624-5175
dc.identifier.piiclockssleep3030034
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/70788
dc.languageeng
dc.publisherMDPI (Basel, Switzerland)
dc.publisher.urihttps://www.mdpi.com/2624-5175/3/3/34
dc.relation.isPartOfClocks Sleep
dc.rights(c) 2021 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectGABA
dc.subjectGABAA receptor
dc.subjectcircadian clock
dc.subjectgeneral anaesthesia
dc.subjectphase response curve
dc.titleThe Effects of General Anaesthesia and Light on Behavioural Rhythms and GABAA Receptor Subunit Expression in the Mouse SCN.
dc.typeJournal article
pubs.elements-id448832
pubs.organisational-groupOther
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