Pilot study on the effects of preservatives on corneal collagen parameters measured by small angle X-ray scattering analysis

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dc.citation.issue1
dc.citation.volume14
dc.contributor.authorKelly SJ
dc.contributor.authorduPlessis L
dc.contributor.authorSoley J
dc.contributor.authorNoble F
dc.contributor.authorWells HC
dc.contributor.authorKelly PJ
dc.coverage.spatialEngland
dc.date.accessioned2023-10-24T21:35:59Z
dc.date.accessioned2023-10-25T20:49:41Z
dc.date.available2021-02-27
dc.date.available2023-10-24T21:35:59Z
dc.date.available2023-10-25T20:49:41Z
dc.date.issued2021-12
dc.date.updated2023-10-24T20:51:13Z
dc.description.abstractOBJECTIVE: Small angle X-ray scattering (SAXS) analysis is a sensitive way of determining the ultrastructure of collagen in tissues. Little is known about how parameters measured by SAXS are affected by preservatives commonly used to prevent autolysis. We determined the effects of formalin, glutaraldehyde, Triton X and saline on measurements of fibril diameter, fibril diameter distribution, and D-spacing of corneal collagen using SAXS analysis. RESULTS: Compared to sections of sheep and cats' corneas stored frozen as controls, those preserved in 5% glutaraldehyde and 10% formalin had significantly larger mean collagen fibril diameters, increased fibril diameter distribution and decreased D-spacing. Sections of corneas preserved in Triton X had significantly increased collagen fibril diameters and decreased fibril diameter distribution. Those preserved in 0.9% saline had significantly increased mean collagen fibril diameters and decreased diameter distributions. Subjectively, the corneas preserved in 5% glutaraldehyde and 10% formalin maintained their transparency but those in Triton X and 0.9% saline became opaque. Subjective morphological assessment of transmission electron microscope images of corneas supported the SAXS data. Workers using SAXS analysis to characterize collagen should be alerted to changes that can be introduced by common preservatives in which their samples may have been stored.
dc.description.confidentialfalse
dc.edition.editionDecember 2021
dc.format.extent78-
dc.identifier78
dc.identifier10.1186/s13104-021-05494-y
dc.identifierhttps://www.ncbi.nlm.nih.gov/pubmed/33640024
dc.identifier.citationKelly SJ, duPlessis L, Soley J, Noble F, Wells HC, Kelly PJ. (2021). Pilot study on the effects of preservatives on corneal collagen parameters measured by small angle X-ray scattering analysis.. BMC Res Notes. 14. 1. (pp. 78-).
dc.identifier.doi10.1186/s13104-021-05494-y
dc.identifier.eissn1756-0500
dc.identifier.elements-typejournal-article
dc.identifier.harvestedMassey_Dark
dc.identifier.issn1756-0500
dc.identifier.urihttp://hdl.handle.net/10179/20406
dc.languageeng
dc.publisherBioMed Central Ltd
dc.publisher.urihttps://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-021-05494-y
dc.relation.isPartOfBMC Res Notes
dc.rights(c) 2021 The Author/sen_US
dc.rightsCC BY 4.0en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subjectCollagen
dc.subjectCornea
dc.subjectFormalin
dc.subjectGlutaraldehyde
dc.subjectSAXS
dc.subjectTritonX
dc.subjectAnimals
dc.subjectCats
dc.subjectCollagen
dc.subjectCornea
dc.subjectPilot Projects
dc.subjectScattering, Small Angle
dc.subjectSheep
dc.subjectX-Ray Diffraction
dc.subjectX-Rays
dc.titlePilot study on the effects of preservatives on corneal collagen parameters measured by small angle X-ray scattering analysis
dc.typeJournal article
pubs.elements-id441123
pubs.organisational-groupOther
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