Unveiling anti-inflammatory peptides from Lion's Mane mushroom (Hericium erinaceus): Preparation, bioactivity assessment, and peptides identification

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dc.citation.issue2
dc.citation.volume5
dc.contributor.authorSilva B
dc.contributor.authorSringarm K
dc.contributor.authorPotikanond S
dc.contributor.authorTangjaidee P
dc.contributor.authorBuacheen P
dc.contributor.authorRachtanapun P
dc.contributor.authorDonlao N
dc.contributor.authorSingh J
dc.contributor.authorKaur L
dc.contributor.authorIssara U
dc.contributor.authorKingwascharapong P
dc.contributor.authorPhongthai S
dc.date.accessioned2025-08-04T23:07:45Z
dc.date.available2025-08-04T23:07:45Z
dc.date.issued2025-12-01
dc.description.abstractLion's Mane mushroom (Hericium erinaceus) is acknowledged worldwide for its substantial contribution of medicinal compounds and nutrients, including protein. The efficient extraction and hydrolysis of proteins are essential for revealing their bioactive properties. This study demonstrates that the optimal pulsed electric field (PEF)-assisted extraction achieved a 42.44 % increase in protein extraction efficiency relative to traditional alkaline extraction (p < 0.05). H. erinaceus protein contained 44.59 % essential amino acids and exhibited 71.33 % in vitro digestibility. Pepsin-trypsin hydrolysis produced the most significant anti-inflammatory activity, resulting in a 36.2 % reduction in nitric oxide and a 31.8 % decrease in interleukin-6 levels (p < 0.05). Subsequent fractionations employing membrane ultrafiltration and size exclusion chromatography effectively purified the peptides, resulting in enhanced anti-inflammatory activity (p < 0.05). This research discovered nine important peptide sequences containing 50–100 % hydrophobic amino acids in Lion's Mane mushroom proteins, which could aid in the synthesis of natural anti-inflammatory peptides.
dc.description.confidentialfalse
dc.edition.editionDecember 2025
dc.identifier.citationSilva B, Sringarm K, Potikanond S, Tangjaidee P, Buacheen P, Rachtanapun P, Donlao N, Singh J, Kaur L, Issara U, Kingwascharapong P, Phongthai S. (2025). Unveiling anti-inflammatory peptides from Lion's Mane mushroom (Hericium erinaceus): Preparation, bioactivity assessment, and peptides identification. Applied Food Research. 5. 2.
dc.identifier.doi10.1016/j.afres.2025.101167
dc.identifier.eissn2772-5022
dc.identifier.elements-typejournal-article
dc.identifier.number101167
dc.identifier.piiS277250222500472X
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/73281
dc.languageEnglish
dc.publisherElsevier B V
dc.publisher.urihttps://www.sciencedirect.com/science/article/pii/S277250222500472X
dc.relation.isPartOfApplied Food Research
dc.rights(c) The author/sen
dc.rights.licenseCC BYen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectAnti-inflammatory peptide
dc.subjectAlternative proteins
dc.subjectLion’s Mane mushroom (Hericium erinaceus)
dc.subjectProtein hydrolysates
dc.subjectIn vitro digestion
dc.titleUnveiling anti-inflammatory peptides from Lion's Mane mushroom (Hericium erinaceus): Preparation, bioactivity assessment, and peptides identification
dc.typeJournal article
pubs.elements-id501687
pubs.organisational-groupOther
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