Effects of omega 3 and omega 6-polyunsaturated fatty acids on RANKL-induced osteoclast differentiation of RAW264.7 cells: A comparative in vitro study

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dc.citation.issue7
dc.citation.volume6
dc.contributor.authorBoeyens JCA
dc.contributor.authorDeepak V
dc.contributor.authorChua W-H
dc.contributor.authorKruger MC
dc.contributor.authorJoubert A
dc.contributor.authorCoetzee M
dc.date.available2014-07-01
dc.date.available2014-06-23
dc.date.issued10/07/2014
dc.descriptionThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.description.abstractPolyunsaturated fatty acids (PUFAs) have been reported to have an anabolic effect on bone in vivo, but comparative studies to identify inhibitors of osteoclast formation amongst ω3- and ω6-PUFAs are still lacking. Here we assessed the effects of the ω3-PUFAs, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and the ω6-PUFAs, arachidonic acid (AA) and γ-linolenic acid (GLA) on a RAW264.7 osteoclast differentiation model. The effects of PUFAs on RANKL-induced osteoclast formation were evaluated by counting tartrate resistant acid phosphatase (TRAP)-positive multinucleated cells. PUFAs significantly inhibited RANKL-induced osteoclast formation in a dose-dependent manner with AA- and DHA-mediated inhibition being the strongest. Furthermore, RANKL-induced mRNA- and protein expression of the key osteoclastogenic genes cathepsin K and TRAP were inhibited by AA and more potently by DHA. Owing to the attenuated osteoclastogenesis by DHA and AA, actin ring formation and bone resorptive activity of these cells as evaluated on bone-mimetic plates were severely compromised. Hence, of the tested PUFAs, AA and DHA were found to be the most effective in inhibiting RANKL-induced osteoclast formation with the latter providing the strongest inhibitory effects. Collectively, the data indicates that these PUFAs may play an important role in regulating bone diseases characterized by excessive osteoclast activity.
dc.description.publication-statusPublished
dc.format.extent2584 - 2601 (18)
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000339989400007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=c5bb3b2499afac691c2e3c1a83ef6fef
dc.identifier.citationNUTRIENTS, 2014, 6 (7), pp. 2584 - 2601 (18)
dc.identifier.doi10.3390/nu6072584
dc.identifier.elements-id213904
dc.identifier.issn2072-6643
dc.identifier.urihttps://hdl.handle.net/10179/9426
dc.languageEnglish
dc.language.isoen
dc.publisherMDPI
dc.relation.isPartOfNUTRIENTS
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectNutrition & Dietetics
dc.subjectNUTRITION & DIETETICS
dc.subjectosteoclasts
dc.subjectpolyunsaturated fatty acids
dc.subjectbone resorption
dc.subjectarachidonic acid
dc.subjectgamma-linolenic acid
dc.subjecteicosapentaenoic acid
dc.subjectdocosahexaenoic acid
dc.subjectRAW264.7 macrophages
dc.subjectALPHA-LINOLENIC ACID
dc.subjectBONE-RESORPTION
dc.subjectDOCOSAHEXAENOIC ACID
dc.subjectCATHEPSIN-K
dc.subjectEICOSAPENTAENOIC ACID
dc.subjectDIETARY RATIO
dc.subjectRESOLVIN E1
dc.subjectINFLAMMATION
dc.subjectMICE
dc.subjectPHOSPHATASE
dc.subject.anzsrc0908 Food Sciences
dc.subject.anzsrc1111 Nutrition and Dietetics
dc.titleEffects of omega 3 and omega 6-polyunsaturated fatty acids on RANKL-induced osteoclast differentiation of RAW264.7 cells: A comparative in vitro study
dc.typeJournal article
pubs.notesNot known
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Health
pubs.organisational-group/Massey University/College of Health/School of Health Science
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