CryoEM structure of the outer membrane secretin channel pIV from the f1 filamentous bacteriophage.

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dc.citation.issue1
dc.citation.volume12
dc.contributor.authorConners R
dc.contributor.authorMcLaren M
dc.contributor.authorŁapińska U
dc.contributor.authorSanders K
dc.contributor.authorStone MRL
dc.contributor.authorBlaskovich MAT
dc.contributor.authorPagliara S
dc.contributor.authorDaum B
dc.contributor.authorRakonjac J
dc.contributor.authorGold VAM
dc.date.available2021-11-02
dc.date.available2021-10-13
dc.date.issued2/11/2021
dc.description.abstractThe Ff family of filamentous bacteriophages infect gram-negative bacteria, but do not cause lysis of their host cell. Instead, new virions are extruded via the phage-encoded pIV protein, which has homology with bacterial secretins. Here, we determine the structure of pIV from the f1 filamentous bacteriophage at 2.7 Å resolution by cryo-electron microscopy, the first near-atomic structure of a phage secretin. Fifteen f1 pIV subunits assemble to form a gated channel in the bacterial outer membrane, with associated soluble domains projecting into the periplasm. We model channel opening and propose a mechanism for phage egress. By single-cell microfluidics experiments, we demonstrate the potential for secretins such as pIV to be used as adjuvants to increase the uptake and efficacy of antibiotics in bacteria. Finally, we compare the f1 pIV structure to its homologues to reveal similarities and differences between phage and bacterial secretins.
dc.description.publication-statusPublished
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000714189200010&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=c5bb3b2499afac691c2e3c1a83ef6fef
dc.identifierARTN 6316
dc.identifier.citationNATURE COMMUNICATIONS, 2021, 12 (1)
dc.identifier.doi10.1038/s41467-021-26610-3
dc.identifier.eissn2041-1723
dc.identifier.elements-id449460
dc.identifier.harvestedMassey_Dark
dc.identifier.urihttps://hdl.handle.net/10179/17418
dc.relation.isPartOfNATURE COMMUNICATIONS
dc.relation.urihttps://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC8563730&blobtype=pdf
dc.titleCryoEM structure of the outer membrane secretin channel pIV from the f1 filamentous bacteriophage.
dc.typeJournal article
pubs.notesNot known
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Sciences
pubs.organisational-group/Massey University/College of Sciences/School of Natural Sciences
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