Comparative genome identification of accessory genes associated with strong biofilm formation in Vibrio parahaemolyticus.

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dc.citation.volume166
dc.contributor.authorWang D
dc.contributor.authorFletcher GC
dc.contributor.authorGagic D
dc.contributor.authorOn SLW
dc.contributor.authorPalmer JS
dc.contributor.authorFlint SH
dc.coverage.spatialCanada
dc.date.accessioned2024-07-16T03:00:18Z
dc.date.available2024-07-16T03:00:18Z
dc.date.issued2023-04-01
dc.description.abstractVibrio parahaemolyticus biofilms on the seafood processing plant surfaces are a potential source of seafood contamination and subsequent food poisoning. Strains differ in their ability to form biofilm, but little is known about the genetic characteristics responsible for biofilm development. In this study, pangenome and comparative genome analysis of V. parahaemolyticus strains reveals genetic attributes and gene repertoire that contribute to robust biofilm formation. The study identified 136 accessory genes that were exclusively present in strong biofilm forming strains and these were functionally assigned to the Gene Ontology (GO) pathways of cellulose biosynthesis, rhamnose metabolic and catabolic processes, UDP-glucose processes and O antigen biosynthesis (p < 0.05). Strategies of CRISPR-Cas defence and MSHA pilus-led attachment were implicated via Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation. Higher levels of horizontal gene transfer (HGT) were inferred to confer more putatively novel properties on biofilm-forming V. parahaemolyticus. Furthermore, cellulose biosynthesis, a neglected potential virulence factor, was identified as being acquired from within the order Vibrionales. The cellulose synthase operons in V. parahaemolyticus were examined for their prevalence (22/138, 15.94 %) and were found to consist of the genes bcsG, bcsE, bcsQ, bcsA, bcsB, bcsZ, bcsC. This study provides insights into robust biofilm formation of V. parahaemolyticus at the genomic level and facilitates: identification of key attributes for robust biofilm formation, elucidation of biofilm formation mechanisms and development of potential targets for novel control strategies of persistent V. parahaemolyticus.
dc.description.confidentialfalse
dc.edition.editionApril 2023
dc.format.pagination112605-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/36914349
dc.identifier.citationWang D, Fletcher GC, Gagic D, On SLW, Palmer JS, Flint SH. (2023). Comparative genome identification of accessory genes associated with strong biofilm formation in Vibrio parahaemolyticus.. Food Res Int. 166. (pp. 112605-).
dc.identifier.doi10.1016/j.foodres.2023.112605
dc.identifier.eissn1873-7145
dc.identifier.elements-typejournal-article
dc.identifier.issn0963-9969
dc.identifier.number112605
dc.identifier.piiS0963-9969(23)00150-3
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/70193
dc.languageeng
dc.publisherElsevier B.V.
dc.publisher.urihttps://www.sciencedirect.com/science/article/pii/S0963996923001503
dc.relation.isPartOfFood Res Int
dc.rights(c) 2023 The Author/s
dc.rightsCC BY-NC-ND 4.0
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCellulose
dc.subjectComparative genome
dc.subjectHorizontal gene transfer (HGT)
dc.subjectSeafood safety
dc.subjectwhole genome sequencing (WGS)
dc.subjectVibrio parahaemolyticus
dc.subjectBiofilms
dc.subjectGenomics
dc.subjectOperon
dc.subjectCellulose
dc.titleComparative genome identification of accessory genes associated with strong biofilm formation in Vibrio parahaemolyticus.
dc.typeJournal article
pubs.elements-id460129
pubs.organisational-groupOther
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