Collagen Fibril Intermolecular Spacing Changes with 2-Propanol: A Mechanism for Tissue Stiffness.

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dc.contributor.authorWells, HCen_US
dc.contributor.authorSizeland, KHen_US
dc.contributor.authorKelly, Sen_US
dc.contributor.authorKirby, Nen_US
dc.contributor.authorHawley, Aen_US
dc.contributor.authorMudie, Sen_US
dc.contributor.authorHaverkamp, RGen_US
dc.date.accessioned2018-05-01T23:43:35Z
dc.date.available2017-08-30en_US
dc.date.available2018-05-01T23:43:35Z
dc.date.issued2017-08-30en_US
dc.description.abstractMaterials composed primarily of collagen are important as surgical scaffolds and other medical devices and require flexibility. However, the factors that control the suppleness and flexibility of these materials are not well understood. Acellular dermal matrix materials in aqueous mixtures of 2-propanol were studied. Synchrotron based small angle X-ray scattering was used to characterize the collagen structure and structural arrangement. Stiffness was measured by bend tests. Bend modulus increased logarithmically with 2-propanol concentration from 0.5 kPa in water to 103 kPa in pure 2-propanol. The intermolecular spacing between tropocollagen molecules decreased from 15.3 to 11.4 Å with increasing 2-propanol concentration while fibril diameter decreased from 57.2 to 37.2 nm. D-spacing initially increased from 63.6 nm to 64.2 nm at 50% 2-propanol then decreased to 60.3 nm in pure 2-propanol. The decrease in intermolecular spacing and fibril diameter are due to removal of water and the collapse of the hydrogen bond structure between tropocollagen molecules causing closer packing of the molecules within a fibril. We speculate this tighter molecular packing may restrict the sliding of collagen within fibrils, and similar disruption of the extended hydration layer between fibrils may lead to restriction of sliding between fibrils. This mechanism for tissue stiffness may be more general.en_US
dc.description.confidentialfalseen_US
dc.identifier.citationACS Biomaterials Science and Engineering, 2017en_US
dc.identifier.doi10.1021/acsbiomaterials.7b00418en_US
dc.identifier.elements-id371813
dc.identifier.harvestedMassey_Dark
dc.identifier.issn2373-9878en_US
dc.identifier.urihttp://hdl.handle.net/10179/13250
dc.publisherAmerican Chemical Societyen_US
dc.relation.isPartOfACS Biomaterials Science and Engineeringen_US
dc.relation.isreplacedby123456789/10769
dc.relation.isreplacedbyhttp://hdl.handle.net/123456789/10769
dc.relation.urihttp://pubs.acs.org/doi/10.1021/acsbiomaterials.7b00418en_US
dc.rightsACS AuthorChoice - This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.en_US
dc.titleCollagen Fibril Intermolecular Spacing Changes with 2-Propanol: A Mechanism for Tissue Stiffness.en_US
dc.typeJournal Article
pubs.notesNot knownen_US
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Sciences
pubs.organisational-group/Massey University/College of Sciences/School of Engineering and Technology
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