Biotransformation of Rutin in In Vitro Porcine Ileal and Colonic Fermentation Models

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dc.citation.issue33
dc.citation.volume71
dc.contributor.authorUlluwishewa D
dc.contributor.authorMontoya CA
dc.contributor.authorMace L
dc.contributor.authorRettedal EA
dc.contributor.authorFraser K
dc.contributor.authorMcNabb WC
dc.contributor.authorMoughan PJ
dc.contributor.authorRoy NC
dc.coverage.spatialUnited States
dc.date.accessioned2024-10-13T21:48:32Z
dc.date.available2024-10-13T21:48:32Z
dc.date.issued2023-08-23
dc.description.abstractQuercetin, a polyphenol antioxidant, is widely distributed in food in the form of glycoside rutin, which is not readily absorbed in the gastrointestinal tract. The microbiota of the colon is known to biotransform rutin, generating quercetin aglycones that can be absorbed. We investigated the role of the ileal and colonic microbiota in rutin biotransformation using established in vitro fermentation models. Overall, a higher rate of rutin biotransformation was observed during colonic fermentation compared with ileal fermentation. The colonic microbiome showed higher potential for rutin conversion to quercetin through an increased abundance of α-rhamnosidase- and β-glucosidase-encoding genes compared to the ileal microbiome. Nonetheless, rutin metabolism occurred rapidly during ileal fermentation (∼20% rutin disappearance after 1 h). The appearance of quercetin varied depending on the ileal inoculum and correlated with an increased abundance of Firmicutes, suggesting that quercetin absorption could be improved via modulation of the ileal microbiota.
dc.description.confidentialfalse
dc.format.pagination12487-12496
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/37578464
dc.identifier.citationUlluwishewa D, Montoya CA, Mace L, Rettedal EA, Fraser K, McNabb WC, Moughan PJ, Roy NC. (2023). Biotransformation of Rutin in In Vitro Porcine Ileal and Colonic Fermentation Models.. J Agric Food Chem. 71. 33. (pp. 12487-12496).
dc.identifier.doi10.1021/acs.jafc.3c00980
dc.identifier.eissn1520-5118
dc.identifier.elements-typejournal-article
dc.identifier.issn0021-8561
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71691
dc.languageeng
dc.publisherAmerican Chemical Society
dc.publisher.urihttps://pubs.acs.org/doi/10.1021/acs.jafc.3c00980
dc.relation.isPartOfJ Agric Food Chem
dc.rights(c) 2023 The Author/s
dc.rightsCC BY-NC-ND 4.0
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectcolon fermentation
dc.subjectfecal microbiome
dc.subjectileal fermentation
dc.subjectileal microbiome
dc.subjectpolyphenol biotransformation
dc.subjectSwine
dc.subjectAnimals
dc.subjectRutin
dc.subjectQuercetin
dc.subjectFermentation
dc.subjectColon
dc.subjectBiotransformation
dc.titleBiotransformation of Rutin in In Vitro Porcine Ileal and Colonic Fermentation Models
dc.typeJournal article
pubs.elements-id479937
pubs.organisational-groupOther
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