The preventive effect of greenshell mussel meat against osteoarthritis in vivo : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Health Science At Massey University, Palmerston North, New Zealand

Simple item page
dc.confidentialEmbargo : Noen_US
dc.contributor.advisorWolber, Fran
dc.contributor.authorSiriarchavatana, Parkpoom
dc.date.accessioned2021-03-29T20:14:54Z
dc.date.accessioned2021-07-20T02:47:09Z
dc.date.available2021-03-29T20:14:54Z
dc.date.available2021-07-20T02:47:09Z
dc.date.issued2021
dc.description.abstractOsteoarthritis (OA) is identified by progressive cartilage erosion of synovial joints. One of the most prevalent OA phenotypes, metabolic OA (MetOA), is linked to metabolic syndrome (MetS). MetS is a combination of obesity, type II diabetes, hypertension, and hyperlipidemia; the effects of these disorders can lead to the development of MetOA. Osteoporosis is characterised by loss of bone mineral density and is causally linked with a decrease in systemic estrogen levels. As MetS, OA and osteoporosis are all prevalent in postmenopausal women, it is possible they may be causally linked. For example, systemic low-grade inflammation in MetS may trigger inflammation in both joints and bone, which could be further aggravated by high fat/high sugar diet (HFHS)-induced obesity and gut dysbiosis. We hypothesized that chronic inflammation would be correlated with MetOA development and therefore decreasing inflammation would be protective. New Zealand greenshell mussel (GSM) contains anti-inflammatory properties shown to reduce OA symptoms and omega-3 fatty acids shown to reduce the development of post-menopausal osteoporosis. We hypothesized GSM could protect against both MetOA and osteoporosis reducing bone resorption, inhibiting inflammation and/or modulating beneficial gut microbes. In vitro, non-polar GSM lipids demonstrated bone-protective properties and significantly reduced osteoclast differentiation, tartrate-resistant acid phosphatase activity, actin ring formation and gene expression of matrix metalloproteinase, cathepsin K, carbonic anhydrase and nuclear factor of activating T cells 1. In vivo, aging, HFHS and OVX produced a rat model mimicking human MetS. Dietary whole GSM powder provided protection by significantly reducing a biomarker of collagen degradation and subsequent joint damage, as well as improving short-term bone mineral density and lean mass accrual. GSM-induced changes in gut microbiota were not correlated with dysbiosis. No changes in inflammatory markers were found, disproving our initial hypothesis and suggesting that chronic inflammation may not be a critical factor in MetOA. In conclusion, GSM as a dietary intervention may reduce the incidence or progression of MetOA but not via altering systemic inflammation or gut dysbiosis.en_US
dc.identifier.urihttp://hdl.handle.net/10179/16523
dc.publisherMassey Universityen_US
dc.rightsThe Authoren_US
dc.subjectOsteoarthritisen
dc.subjectPreventionen
dc.subjectAlternative treatmenten
dc.subjectPernaen
dc.subjectTherapeutic useen
dc.subjectDiseasesen
dc.subjectAnimal models
dc.subject.anzsrc320223 Rheumatology and arthritisen
dc.titleThe preventive effect of greenshell mussel meat against osteoarthritis in vivo : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Health Science At Massey University, Palmerston North, New Zealanden_US
dc.typeThesisen_US
massey.contributor.authorSiriarchavatana, Parkpoomen_US
thesis.degree.disciplineHealth Scienceen_US
thesis.degree.grantorMassey Universityen_US
thesis.degree.levelDoctoralen_US
thesis.degree.nameDoctor of Philosophy (PhD)en_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
SiriarchavatanaPhDThesis.pdf
Size:
7.55 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Theses and Dissertations