Endoplasmic Reticulum Stress and Autophagy Markers in Soleus Muscle Disuse-Induced Atrophy of Rats Treated with Fish Oil

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dc.citation.issue7
dc.citation.volume13
dc.contributor.authorMarzuca-Nassr GN
dc.contributor.authorKuwabara WMT
dc.contributor.authorVitzel KF
dc.contributor.authorMurata GM
dc.contributor.authorTorres RP
dc.contributor.authorMancini-Filho J
dc.contributor.authorAlba-Loureiro TC
dc.contributor.authorCuri R
dc.coverage.spatialSwitzerland
dc.date.accessioned2023-08-10T22:40:56Z
dc.date.accessioned2023-09-04T01:41:07Z
dc.date.available2021-07-03
dc.date.available2023-08-10T22:40:56Z
dc.date.available2023-09-04T01:41:07Z
dc.date.issued2021-07
dc.date.updated2023-08-10T22:35:49Z
dc.description.abstractEndoplasmic reticulum stress (ERS) and autophagy pathways are implicated in disuse muscle atrophy. The effects of high eicosapentaenoic (EPA) or high docosahexaenoic (DHA) fish oils on soleus muscle ERS and autophagy markers were investigated in a rat hindlimb suspension (HS) atrophy model. Adult Wistar male rats received daily by gavage supplementation (0.3 mL per 100 g b.w.) of mineral oil or high EPA or high DHA fish oils (FOs) for two weeks. Afterward, the rats were subjected to HS and the respective treatments concomitantly for an additional two-week period. After four weeks, we evaluated ERS and autophagy markers in the soleus muscle. Results were analyzed using two-way analysis of variance (ANOVA) and Bonferroni post hoc test. Gastrocnemius muscle ω-6/ω-3 fatty acids (FAs) ratio was decreased by both FOs indicating the tissue incorporation of omega-3 fatty acids. HS altered (p < 0.05) the protein content (decreasing total p38 and BiP and increasing p-JNK2/total JNK2 ratio, and caspase 3) and gene expressions (decreasing BiP and increasing IRE1 and PERK) of ERS and autophagy (decreasing Beclin and increasing LC3 and ATG14) markers in soleus. Both FOs attenuated (p < 0.05) the increase in PERK and ATG14 expressions induced by HS. Thus, both FOs could potentially attenuate ERS and autophagy in skeletal muscles undergoing atrophy.
dc.format.extent2298-
dc.identifierARTN 2298
dc.identifiernu13072298
dc.identifierhttps://www.ncbi.nlm.nih.gov/pubmed/34371808
dc.identifier.citationMarzuca-Nassr GN, Kuwabara WMT, Vitzel KF, Murata GM, Torres RP, Mancini-Filho J, Alba-Loureiro TC, Curi R. (2021). Endoplasmic Reticulum Stress and Autophagy Markers in Soleus Muscle Disuse-Induced Atrophy of Rats Treated with Fish Oil.. Nutrients. 13. 7. (pp. 2298-).
dc.identifier.doi10.3390/nu13072298
dc.identifier.eissn2072-6643
dc.identifier.elements-typejournal-article
dc.identifier.harvestedMassey_Dark
dc.identifier.issn2072-6643
dc.identifier.urihttp://hdl.handle.net/10179/19924
dc.languageeng
dc.publisherMDPI (Basel, Switzerland)
dc.relation.isPartOfNutrients
dc.rightsCopyright: © 2021 by the authors
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectdocosahexaenoic acid
dc.subjecteicosapentanoic acid
dc.subjecthindlimb suspension
dc.subjectskeletal muscle atrophy
dc.subjectunfolded protein response
dc.subjectω-3 fatty acids
dc.subjectAnimals
dc.subjectAutophagy
dc.subjectBiomarkers
dc.subjectDisease Models, Animal
dc.subjectDocosahexaenoic Acids
dc.subjectEicosapentaenoic Acid
dc.subjectEndoplasmic Reticulum Stress
dc.subjectFish Oils
dc.subjectHindlimb Suspension
dc.subjectMale
dc.subjectMuscle, Skeletal
dc.subjectMuscular Atrophy
dc.subjectRats
dc.subjectRats, Wistar
dc.titleEndoplasmic Reticulum Stress and Autophagy Markers in Soleus Muscle Disuse-Induced Atrophy of Rats Treated with Fish Oil
dc.typeJournal article
pubs.elements-id447182
pubs.organisational-groupOther
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