In vivo transcriptome analysis provides insights into host-dependent expression of virulence factors by Yersinia entomophaga MH96, during infection of Galleria mellonella

dc.citation.issue1
dc.citation.volume11
dc.contributor.authorPaulson AR
dc.contributor.authorO'Callaghan M
dc.contributor.authorZhang X-X
dc.contributor.authorRainey PB
dc.contributor.authorHurst MRH
dc.contributor.editorOliver B
dc.coverage.spatialEngland
dc.date.accessioned2024-02-08T22:25:50Z
dc.date.accessioned2024-07-25T06:46:13Z
dc.date.available2020-12-08
dc.date.available2024-02-08T22:25:50Z
dc.date.available2024-07-25T06:46:13Z
dc.date.issued2021-01
dc.description.abstractThe function of microbes can be inferred from knowledge of genes specifically expressed in natural environments. Here, we report the in vivo transcriptome of the entomopathogenic bacterium Yersinia entomophaga MH96, captured during initial, septicemic, and pre-cadaveric stages of intrahemocoelic infection in Galleria mellonella. A total of 1285 genes were significantly upregulated by MH96 during infection; 829 genes responded to in vivo conditions during at least one stage of infection, 289 responded during two stages of infection, and 167 transcripts responded throughout all three stages of infection compared to in vitro conditions at equivalent cell densities. Genes upregulated during the earliest infection stage included components of the insecticidal toxin complex Yen-TC (chi1, chi2, and yenC1), genes for rearrangement hotspot element containing protein yenC3, cytolethal distending toxin cdtAB, and vegetative insecticidal toxin vip2. Genes more highly expressed throughout the infection cycle included the putative heat-stable enterotoxin yenT and three adhesins (usher-chaperone fimbria, filamentous hemagglutinin, and an AidA-like secreted adhesin). Clustering and functional enrichment of gene expression data also revealed expression of genes encoding type III and VI secretion system-associated effectors. Together these data provide insight into the pathobiology of MH96 and serve as an important resource supporting efforts to identify novel insecticidal agents.
dc.description.confidentialfalse
dc.edition.editionJanuary 2021
dc.format.paginationjkaa024-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/33561230
dc.identifier.citationPaulson AR, O'Callaghan M, Zhang X-X, Rainey PB, Hurst MRH. (2021). In vivo transcriptome analysis provides insights into host-dependent expression of virulence factors by Yersinia entomophaga MH96, during infection of Galleria mellonella.. G3 (Bethesda). 11. 1. (pp. jkaa024-).
dc.identifier.doi10.1093/g3journal/jkaa024
dc.identifier.eissn2160-1836
dc.identifier.elements-typejournal-article
dc.identifier.issn2160-1836
dc.identifier.numberARTN jkaa024
dc.identifier.pii6026962
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/70834
dc.languageeng
dc.publisherOxford University Press on behalf of Genetics Society of America
dc.publisher.urihttps://academic.oup.com/g3journal/article/11/1/jkaa024/6026962
dc.relation.isPartOfG3 (Bethesda)
dc.rights(c) 2020 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectGalleria mellonella
dc.subjectYersinia virulence factors
dc.subjectentomopathogenic bacteria
dc.subjectinsecticidal toxins
dc.subjecttranscriptome
dc.subjectAnimals
dc.subjectGene Expression Profiling
dc.subjectHumans
dc.subjectMoths
dc.subjectVirulence Factors
dc.subjectYersinia
dc.titleIn vivo transcriptome analysis provides insights into host-dependent expression of virulence factors by Yersinia entomophaga MH96, during infection of Galleria mellonella
dc.typeJournal article
pubs.elements-id440087
pubs.organisational-groupOther
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