In silico resurrection of the major vault protein suggests it is ancestral in modern eukaryotes

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dc.citation.issue8
dc.citation.volume5
dc.contributor.authorDaly TK
dc.contributor.authorSutherland-Smith AJ
dc.contributor.authorPenny ED
dc.date.available2013-01-01
dc.date.issued2013-07-25
dc.description(c) The Author(s) 2013
dc.description.abstractVaults are very large oligomeric ribonucleoproteins conserved among a variety of species. The rat vault 3D structure shows an ovoid oligomeric particle, consisting of 78 major vault protein monomers, each of approximately 861 amino acids. Vaults are probably the largest ribonucleoprotein structures in eukaryote cells, being approximately 70 nm in length with a diameter of 40 nm--the size of three ribosomes and with a lumen capacity of 50 million Å(3). We use both protein sequences and inferred ancestral sequences for in silico virtual resurrection of tertiary and quaternary structures to search for vaults in a wide variety of eukaryotes. We find that the vault's phylogenetic distribution is widespread in eukaryotes, but is apparently absent in some notable model organisms. Our conclusion from the distribution of vaults is that they were present in the last eukaryote common ancestor but they have apparently been lost from a number of groups including fungi, insects, and probably plants. Our approach of inferring ancestral 3D and quaternary structures is expected to be useful generally.
dc.description.publication-statusPublished
dc.format.extent1567 - 1583 (17)
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000324595000010&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=c5bb3b2499afac691c2e3c1a83ef6fef
dc.identifier.citationGENOME BIOLOGY AND EVOLUTION, 2013, 5 (8), pp. 1567 - 1583 (17)
dc.identifier.doi10.1093/gbe/evt113
dc.identifier.elements-id193955
dc.identifier.harvestedMassey_Dark
dc.identifier.issn1759-6653
dc.languageEnglish
dc.publisherOxford University Press
dc.relation.isPartOfGENOME BIOLOGY AND EVOLUTION
dc.rightsCC BY-NC 3.0
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectEvolutionary Biology
dc.subjectGenetics & Heredity
dc.subjectEVOLUTIONARY BIOLOGY
dc.subjectGENETICS & HEREDITY
dc.subjectvault ribonucleoprotein
dc.subjectancestral reconstruction (ASR)
dc.subjectBLAST
dc.subjectI-TASSER
dc.subjectRosettaDock
dc.subjectlast eukaryotic common ancestor
dc.subjectSTRUCTURE PREDICTION
dc.subjectEVOLUTIONARY HISTORY
dc.subjectMAXIMUM-LIKELIHOOD
dc.subjectSERVER
dc.subjectTREE
dc.subjectSEQUENCE
dc.subjectORIGIN
dc.subjectPHYLOGENOMICS
dc.subjectCONSERVATION
dc.subjectINSIGHTS
dc.subject.anzsrc0601 Biochemistry and Cell Biology
dc.subject.anzsrc0603 Evolutionary Biology
dc.subject.anzsrc0604 Genetics
dc.titleIn silico resurrection of the major vault protein suggests it is ancestral in modern eukaryotes
dc.typeJournal article
pubs.notesNot known
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Sciences
pubs.organisational-group/Massey University/College of Sciences/School of Natural Sciences
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