Impact of a High Protein Intake on the Plasma Metabolome in Elderly Males: 10 Week Randomized Dietary Intervention

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dc.citation.volume6
dc.contributor.authorDurainayagam B
dc.contributor.authorMitchell CJ
dc.contributor.authorMilan AM
dc.contributor.authorZeng N
dc.contributor.authorSharma P
dc.contributor.authorMitchell SM
dc.contributor.authorRamzan F
dc.contributor.authorKnowles SO
dc.contributor.authorSjödin A
dc.contributor.authorWagner K-H
dc.contributor.authorRoy NC
dc.contributor.authorFraser K
dc.contributor.authorCameron-Smith D
dc.coverage.spatialSwitzerland
dc.date.accessioned2024-10-22T02:15:56Z
dc.date.available2024-10-22T02:15:56Z
dc.date.issued2019-12-06
dc.description.abstractHigh protein diets may improve the maintenance of skeletal muscle mass in the elderly, although it remains less clear what broader impact such diets have on whole body metabolic regulation in the elderly. Non-targeted polar metabolomics analysis using HILIC HPLC-MS was used to profile the circulating plasma metabolome of elderly men (n = 31; 74.7 ± 4.0 years) who were randomized to consume for 10 weeks a diet designed to achieve either protein (RDA; 0.8·g-1·kg-1) or that doubled this recommend intake (2RDA; 1.6.g.kg-1). A limited number of plasma metabolites (n = 24) were significantly differentially regulated by the diet. These included markers of protein anabolism, which increased by the 2RDA diet, including; urea, creatine, and glutarylcarnitine. Whilst in response to the RDA diet; glutamine, glutamic acid, and proline were increased, relative to the 2RDA diet (p < 0.05). Metaboanalyst identified six major metabolic pathways to be influenced by the quantity of protein intake, most notably the arginine and proline pathways. Doubling of the recommended protein intake in older males over 10 weeks exerted only a limited impact on circulating metabolites, as determined by LC-MS. This metabolomic response was almost entirely due to increased circulating abundances of metabolites potentially indicative of altered protein anabolism, without evidence of impact on pathways for metabolic health. Trial Registration: This trial was registered on 3rd March 2016 at the Australia New Zealand Clinical Trial Registry (www.anzctr.org.au) at ACTRN 12616000310460.
dc.description.confidentialfalse
dc.format.pagination180-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/31867339
dc.identifier.citationDurainayagam B, Mitchell CJ, Milan AM, Zeng N, Sharma P, Mitchell SM, Ramzan F, Knowles SO, Sjödin A, Wagner K-H, Roy NC, Fraser K, Cameron-Smith D. (2019). Impact of a High Protein Intake on the Plasma Metabolome in Elderly Males: 10 Week Randomized Dietary Intervention.. Front Nutr. 6. (pp. 180-).
dc.identifier.doi10.3389/fnut.2019.00180
dc.identifier.eissn2296-861X
dc.identifier.elements-typejournal-article
dc.identifier.issn2296-861X
dc.identifier.numberARTN 180
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71798
dc.languageeng
dc.publisherFrontiers Media S.A.
dc.publisher.urihttps://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2019.00180/full
dc.relation.isPartOfFront Nutr
dc.rights(c) 2019 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectdietary protein
dc.subjectnutritional interventions
dc.subjectolder adults
dc.subjectpathway mapping
dc.subjectplasma metabolomics
dc.titleImpact of a High Protein Intake on the Plasma Metabolome in Elderly Males: 10 Week Randomized Dietary Intervention
dc.typeJournal article
pubs.elements-id434465
pubs.organisational-groupOther
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