Arachidonic acid and docosahexaenoic acid suppress osteoclast formation and activity in human CD14+ monocytes, in vitro.

Simple item page
dc.citation.issue4
dc.citation.volume10
dc.contributor.authorKasonga AE
dc.contributor.authorDeepak V
dc.contributor.authorKruger MC
dc.contributor.authorCoetzee M
dc.date.available2015-04-13
dc.date.available2015-03-14
dc.date.issued2015
dc.descriptionThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.description.abstractAn unbalanced diet can have adverse effects on health. Long chain polyunsaturated fatty acids (LCPUFAs) have been the focus of research owing to their necessity of inclusion in a healthy diet. However, the effects of LCPUFAs on human osteoclast formation and function have not been explored before. A human CD14+ monocyte differentiation model was used to elucidate the effects of an ω-3 LCPUFA, docosahexaenoic acid (DHA), and an ω-6 LCPUFA, arachidonic acid (AA), on osteoclast formation and activity. CD14+ monocytes were isolated from peripheral blood of healthy donors and stimulated with macrophage colony stimulating factor and receptor activator of nuclear factor kappa-B ligand to generate osteoclasts. Data from this study revealed that both the LCPUFAs decreased osteoclast formation potential of CD14+ monocytes in a dose-dependent manner when treated at an early stage of differentiation. Moreover, when exposed at a late stage of osteoclast differentiation AA and DHA impaired the bone resorptive potential of mature osteoclasts without affecting osteoclast numbers. AA and DHA abrogated vitronectin receptor expression in differentiating as well as mature osteoclasts. In contrast, the degree of inhibition for calcitonin receptor expression varied between the LCPUFAs with only AA causing inhibition during osteoclast differentiation. Furthermore, AA and DHA down regulated the expression of key osteoclast-specific genes in differentiating as well as mature osteoclasts. This study demonstrates for the first time that LCPUFAs can modulate osteoclast formation and function in a human primary osteoclast cell line.
dc.description.publication-statusPublished
dc.format.extent? - ? (19)
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000352845100292&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=c5bb3b2499afac691c2e3c1a83ef6fef
dc.identifierARTN e0125145
dc.identifier.citationPLOS ONE, 2015, 10 (4), pp. ? - ? (19)
dc.identifier.doi10.1371/journal.pone.0125145
dc.identifier.elements-id231666
dc.identifier.harvestedMassey_Dark
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/10179/8068
dc.languageEnglish
dc.publisherPUBLIC LIBRARY SCIENCE
dc.relation.isPartOfPLOS ONE
dc.subjectScience & Technology
dc.subjectMultidisciplinary Sciences
dc.subjectScience & Technology - Other Topics
dc.subjectN-3 FATTY-ACIDS
dc.subjectBONE-MINERAL DENSITY
dc.subjectEICOSAPENTAENOIC ACID
dc.subjectGENE-EXPRESSION
dc.subjectPHOSPHATASE 5B
dc.subjectHIP FRACTURE
dc.subjectCATHEPSIN K
dc.subjectDIFFERENTIATION
dc.subjectRISK
dc.subjectOMEGA-3-FATTY-ACIDS
dc.titleArachidonic acid and docosahexaenoic acid suppress osteoclast formation and activity in human CD14+ monocytes, in vitro.
dc.typeJournal article
pubs.notesNot known
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Health
pubs.organisational-group/Massey University/College of Health/School of Health Science
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Arachidonic Acid and Docosahexaenoic Acid Suppress Osteoclast Formation and Activity in Human CD14+ Monocytes, In vitro..pdf
Size:
8.33 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Journal Articles