Nitrous Oxide Treatment after Pollination Induces Ploidy Changes in Statice (Limonium sp.)

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dc.citation.issue8
dc.citation.volume10
dc.contributor.authorCordoba-Sanchez J
dc.contributor.authorFunnell K
dc.contributor.authorHedderley D
dc.contributor.authorRoskruge N
dc.contributor.authorMorgan E
dc.contributor.editorNeri D
dc.date.accessioned2024-10-30T18:35:21Z
dc.date.available2024-10-30T18:35:21Z
dc.date.issued2024-08-01
dc.description.abstractThe production of statice (Limonium sp.) plants with higher ploidy through induction of whole-genome duplication (WGD) via the spindle disrupter nitrous oxide (N2O) was examined as a strategy to increase the germplasm diversity of the species. Furthermore, the impact of the resulting ploidy changes on the morphological features of the progeny was examined. Intraspecific crosses between diploid plants of Limonium sinuatum (L.) Mill and L. perezii (Stapf) Hubb. were conducted daily for seven consecutive days, with subsequent exposure to N2O. Within the resulting progeny, between 16% and 35% of plants were polyploid when N2O was applied between one and four days after pollination. A comparative analysis between diploid and tetraploid progeny was conducted, using a selection of 10 L. sinuatum (5 diploids and 5 tetraploids) and 7 L. perezii (4 diploids and 3 tetraploids) genotypes. The results revealed differences between tetraploids and their diploid counterparts for most of the evaluated characteristics. Tetraploid plants of L. sinuatum and L. perezii exhibited pollen grains 1.5 times larger in plan area; the leaves and main floral stem diameter were 1.2 and 1.5 times thicker for L. sinuatum and L. perezii, respectively, the guard cell length was 1.4 times greater for both species, while the stomatal density was 0.6 times lower for L. perezii and 0.8 for L. sinuatum. The leaf area and main floral stem wings were affected by the ploidy increase only for L. sinuatum. In this regard, tetraploid plants of L. sinuatum displayed leaves 1.8 times bigger and main floral steam wings 2.4 times wider in comparison to diploid plants. In conclusion, the production of tetraploid Limonium plants using N2O shortly after pollination creates new diversity for breeding. These findings underscore the potential for leveraging polyploidy as a strategy to enhance desirable traits in Limonium species.
dc.description.confidentialfalse
dc.edition.editionAugust 2024
dc.identifier.citationCordoba-Sanchez J, Funnell K, Hedderley D, Roskruge N, Morgan E. (2024). Nitrous Oxide Treatment after Pollination Induces Ploidy Changes in Statice (Limonium sp.). Horticulturae. 10. 8.
dc.identifier.doi10.3390/horticulturae10080816
dc.identifier.eissn2311-7524
dc.identifier.elements-typejournal-article
dc.identifier.number816
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71870
dc.languageEnglish
dc.publisherMDPI (Basel, Switzerland)
dc.publisher.urihttps://www.mdpi.com/2311-7524/10/8/816
dc.relation.isPartOfHorticulturae
dc.rights(c) 2024 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectautopolyploid
dc.subjectgene dosage effect
dc.subjectmitotic disruption
dc.subjectornamental plants
dc.subjectpolyploidization
dc.subjectwhole-genome duplication
dc.titleNitrous Oxide Treatment after Pollination Induces Ploidy Changes in Statice (Limonium sp.)
dc.typeJournal article
pubs.elements-id491607
pubs.organisational-groupCollege of Health
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