Detection of Foot-and-Mouth Disease Virus in the Absence of Clinical Disease in Cattle and Buffalo in South East Asia

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dc.citation.volume8
dc.contributor.authorBuckle K
dc.contributor.authorBueno R
dc.contributor.authorMcFadden A
dc.contributor.authorvan Andel M
dc.contributor.authorSpence R
dc.contributor.authorHamill C
dc.contributor.authorRoe W
dc.contributor.authorVallee E
dc.contributor.authorCastillo-Alcala F
dc.contributor.authorAbila R
dc.contributor.authorVerin B
dc.contributor.authorPurevsuren B
dc.contributor.authorSutar A
dc.contributor.authorWin HH
dc.contributor.authorThiha M
dc.contributor.authorLwin KO
dc.contributor.authorKhounsy S
dc.contributor.authorPhonthasy S
dc.contributor.authorSouriya V
dc.contributor.authorKeokhamphet C
dc.contributor.authorArzt J
dc.contributor.authorLudi A
dc.contributor.authorMioulet V
dc.contributor.editorCapozzo AV
dc.coverage.spatialSwitzerland
dc.date.accessioned2024-01-17T21:49:39Z
dc.date.accessioned2024-07-25T06:37:56Z
dc.date.available2021-07-23
dc.date.available2024-01-17T21:49:39Z
dc.date.available2024-07-25T06:37:56Z
dc.date.issued2021-07-23
dc.description.abstractFoot-and-mouth disease virus (FMDV) is widespread throughout much of the world, including parts of South East Asia. Surveillance is often limited in endemic areas, relying predominantly on passive outbreak reporting. As part of the World Organisation for Animal Health (OIE)'s South East Asia and China Foot-and-Mouth Disease Project (SEACFMD), field sampling was performed to help understand evidence of widespread virus exposure observed in previous studies. Serum and dry mucosal swabs were collected to evaluate the presence of FMDV RNA on the nasal, oral, and dorsal nasopharyngeal mucosal surfaces of 262 healthy cattle (n = 84 in Laos; n = 125 in Myanmar) and buffalo (n = 48 in Laos; n = 5 in Myanmar) immediately following slaughter in three slaughterhouses. Swabs and serum were tested by the OIE/FAO World Reference Laboratory for foot-and-mouth disease (WRLFMD) using pan-serotypic real-time reverse transcription-PCR (rRT-PCR) and serum was evaluated using the FMD PrioCHECK non-structural protein (NSP) ELISA. In total, 7.3% of animals had detectable FMDV RNA in one or more of the three sites including 5.3% of nasopharyngeal swabs, 2.3% of oral swabs, and 1.5% of nasal swabs. No FMDV RNA was detected in serum. Overall, 37.8% of animals were positive for NSP antibodies, indicating likely past natural exposure to FMDV. Results were comparable for Laos and Myanmar, and for both cattle and buffalo, and were not significantly different between age groups. Detectable FMDV RNA present on the oral and nasal mucosa of clinically-healthy large ruminants in Laos and Myanmar demonstrates the importance of sampling asymptomatic animals as part of surveillance, and may indicate that subclinical infection plays a role in the epidemiology of FMD in these countries.
dc.description.confidentialfalse
dc.format.pagination691308-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/34368278
dc.identifier.citationBuckle K, Bueno R, McFadden A, van Andel M, Spence R, Hamill C, Roe W, Vallee E, Castillo-Alcala F, Abila R, Verin B, Purevsuren B, Sutar A, Win HH, Thiha M, Lwin KO, Khounsy S, Phonthasy S, Souriya V, Keokhamphet C, Arzt J, Ludi A, Mioulet V. (2021). Detection of Foot-and-Mouth Disease Virus in the Absence of Clinical Disease in Cattle and Buffalo in South East Asia.. Front Vet Sci. 8. (pp. 691308-).
dc.identifier.doi10.3389/fvets.2021.691308
dc.identifier.eissn2297-1769
dc.identifier.elements-typejournal-article
dc.identifier.issn2297-1769
dc.identifier.numberARTN 691308
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/70582
dc.languageeng
dc.publisherFrontiers Media S.A.
dc.publisher.urihttps://www.frontiersin.org/articles/10.3389/fvets.2021.691308/full
dc.relation.isPartOfFront Vet Sci
dc.rights(c) 2021 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectabattoirs
dc.subjectbuffaloes
dc.subjectcattle
dc.subjectfoot-and-mouth disease
dc.subjectfoot-and-mouth disease virus
dc.subjectreverse transcriptase polymerase chain reaction
dc.subjectsurveillance
dc.titleDetection of Foot-and-Mouth Disease Virus in the Absence of Clinical Disease in Cattle and Buffalo in South East Asia
dc.typeJournal article
pubs.elements-id447975
pubs.organisational-groupOther
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