Effects of whey protein treatment in an in vitro intestinal cell model following oxidative stress or inflammatory challenge

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dc.citation.volume164
dc.contributor.authorWillems E
dc.contributor.authorPurba A
dc.contributor.authorSavoian MS
dc.contributor.authorHefer C
dc.contributor.authorMaes E
dc.contributor.authorUlluwishewa D
dc.date.accessioned2025-02-19T01:58:34Z
dc.date.available2025-02-19T01:58:34Z
dc.date.issued2025-01-28
dc.description.abstractBovine milk whey proteins with an isoelectric point >6.8 (‘whey’) have demonstrated anti-inflammatory and antioxidant properties. In the present study, pre-treatment of human intestinal cells (Caco-2) with whey mitigated intracellular reactive oxygen species produced in response to the pro-oxidant 2,2′-azobis (2-methylpropionamide)-dihydrochloride (AAPH). The mitigating effect was dose-dependent, and persisted when whey was removed prior to the addition of AAPH. Whey treatment also improved transepithelial electrical resistance (TEER), but returned to untreated-control levels upon removal of whey. Hence, whey can lead to cellular adaptations that aid intestinal function, but can exert additional properties while in contact with cells. Confocal imaging indicated that the previously observed TEER improvements in inflammatory-challenged Caco-2 monolayers were not due to the localisation of occludin or zonula occludens-1 tight junction proteins. However, proteomics analysis indicated a role for other tight junction proteins and provided insights into cellular adaptations that occur in response to whey pre-treatment.
dc.description.confidentialfalse
dc.edition.editionMay 2025
dc.identifier.citationWillems E, Purba A, Savoian MS, Hefer C, Maes E, Ulluwishewa D. (2025). Effects of whey protein treatment in an in vitro intestinal cell model following oxidative stress or inflammatory challenge. International Dairy Journal. 164.
dc.identifier.doi10.1016/j.idairyj.2025.106187
dc.identifier.elements-typejournal-article
dc.identifier.issn0958-6946
dc.identifier.number106187
dc.identifier.piiS0958694625000068
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/72510
dc.languageEnglish
dc.publisherElsevier Ltd
dc.publisher.urihttps://www.sciencedirect.com/science/article/pii/S0958694625000068
dc.relation.isPartOfInternational Dairy Journal
dc.rights(c) 2025 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleEffects of whey protein treatment in an in vitro intestinal cell model following oxidative stress or inflammatory challenge
dc.typeJournal article
pubs.elements-id499529
pubs.organisational-groupOther
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