Evolutionary history of rabies in Ghana

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dc.citation.issue4
dc.citation.volume5
dc.contributor.authorHayman DTS
dc.contributor.authorJohnson N
dc.contributor.authorHorton DL
dc.contributor.authorHedge J
dc.contributor.authorWakeley PR
dc.contributor.authorBanyard AC
dc.contributor.authorZhang S
dc.contributor.authorAlhassan A
dc.contributor.authorFooks AR
dc.date.available2011-04
dc.date.available2011-03-07
dc.date.issued2011
dc.description.abstractRabies virus (RABV) is enzootic throughout Africa, with the domestic dog (Canis familiaris) being the principal vector. Dog rabies is estimated to cause 24,000 human deaths per year in Africa, however, this estimate is still considered to be conservative. Two sub-Saharan African RABV lineages have been detected in West Africa. Lineage 2 is present throughout West Africa, whereas Africa 1a dominates in northern and eastern Africa, but has been detected in Nigeria and Gabon, and Africa 1b was previously absent from West Africa. We confirmed the presence of RABV in a cohort of 76 brain samples obtained from rabid animals in Ghana collected over an eighteen-month period (2007-2009). Phylogenetic analysis of the sequences obtained confirmed all viruses to be RABV, belonging to lineages previously detected in sub-Saharan Africa. However, unlike earlier reported studies that suggested a single lineage (Africa 2) circulates in West Africa, we identified viruses belonging to the Africa 2 lineage and both Africa 1 (a and b) sub-lineages. Phylogeographic Bayesian Markov chain Monte Carlo analysis of a 405 bp fragment of the RABV nucleoprotein gene from the 76 new sequences derived from Ghanaian animals suggest that within the Africa 2 lineage three clades co-circulate with their origins in other West African countries. Africa 1a is probably a western extension of a clade circulating in central Africa and the Africa 1b virus a probable recent introduction from eastern Africa. We also developed and tested a novel reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of RABV in African laboratories. This RT-LAMP was shown to detect both Africa 1 and 2 viruses, including its adaptation to a lateral flow device format for product visualization. These data suggest that RABV epidemiology is more complex than previously thought in West Africa and that there have been repeated introductions of RABV into Ghana. This analysis highlights the potential problems of individual developing nations implementing rabies control programmes in the absence of a regional programme.
dc.description.publication-statusPublished
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000289937400024&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=c5bb3b2499afac691c2e3c1a83ef6fef
dc.identifierARTN e1001
dc.identifier.citationPLOS NEGLECTED TROPICAL DISEASES, 2011, 5 (4)
dc.identifier.doi10.1371/journal.pntd.0001001
dc.identifier.elements-id220096
dc.identifier.harvestedMassey_Dark
dc.identifier.issn1935-2735
dc.identifier.urihttps://hdl.handle.net/10179/7459
dc.publisherPublic Library of Science
dc.relation.isPartOfPLOS NEGLECTED TROPICAL DISEASES
dc.subject.anzsrc06 Biological Sciences
dc.subject.anzsrc11 Medical and Health Sciences
dc.titleEvolutionary history of rabies in Ghana
dc.typeJournal article
pubs.notesNot known
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Sciences
pubs.organisational-group/Massey University/College of Sciences/School of Veterinary Science
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