Probing the Phytochemical Composition and Antioxidant Activity of Moringa oleifera under Ideal Germination Conditions

Simple item page
dc.citation.issue16
dc.citation.volume12
dc.contributor.authorBhuker A
dc.contributor.authorMalik A
dc.contributor.authorPunia H
dc.contributor.authorMcGill C
dc.contributor.authorSofkova-Bobcheva S
dc.contributor.authorMor VS
dc.contributor.authorSingh N
dc.contributor.authorAhmad A
dc.contributor.authorMansoor S
dc.contributor.editorSetzer WN
dc.coverage.spatialSwitzerland
dc.date.accessioned2024-11-06T21:22:19Z
dc.date.available2024-11-06T21:22:19Z
dc.date.issued2023-08-21
dc.description.abstractMoringa oleifera is a rich source of polyphenols whose contents and profile may vary according to environmental conditions, harvest season, and plant tissue. The present study aimed to characterize the profile of phenolic compounds in different tissues of M. oleifera grown under different temperatures (25, 30, and 35 °C), using HPLC/MS, as well as their constituent phytochemicals and in vitro antioxidant activities. The in vitro antioxidant activity of the extracts was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis-3-ethylenebenzothiozoline-6-sulfonicacid (ABTS), and ferric-reducing antioxidant power (FRAP) methods. The polyphenolic compounds were mainly found in the leaves at 30 °C. UPLC/QTOF-MS allowed for the identification of 34 polyphenolic components in seedlings, primarily consisting of glucosides, phenols, flavonoids, and methoxy flavones. At 30 °C, the specific activities of antioxidative enzymes were the highest in leaves, followed by seedlings and then seeds. The leaf and seed extracts also exhibited a greater accumulation of proline, glycine betaine, and antioxidants, such as ascorbic acid, and carotenoids, as measured by the inhibition of ROS production. We found that changes in the expression levels of the validated candidate genes Cu/Zn-SOD, APX, GPP, and TPS lead to significant differences in the germination rate and biochemical changes. These findings demonstrate that M. oleifera plants have high concentrations of phytochemicals and antioxidants, making them an excellent choice for further research to determine their use as health-promoting dietary supplements.
dc.description.confidentialfalse
dc.edition.editionAugust 2023
dc.format.pagination3010-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/37631221
dc.identifier.citationBhuker A, Malik A, Punia H, McGill C, Sofkova-Bobcheva S, Mor VS, Singh N, Ahmad A, Mansoor S. (2023). Probing the Phytochemical Composition and Antioxidant Activity of Moringa oleifera under Ideal Germination Conditions.. Plants (Basel). 12. 16. (pp. 3010-).
dc.identifier.doi10.3390/plants12163010
dc.identifier.eissn2223-7747
dc.identifier.elements-typejournal-article
dc.identifier.issn2223-7747
dc.identifier.number3010
dc.identifier.piiplants12163010
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71945
dc.languageeng
dc.publisherMDPI (Basel, Switzerland)
dc.publisher.urihttps://www.mdpi.com/2223-7747/12/16/3010
dc.relation.isPartOfPlants (Basel)
dc.rights(c) 2023 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectMoringa oleifera
dc.subjectantioxidants
dc.subjectbiochemical analysis
dc.subjectmedicinal plant
dc.subjectphenolics
dc.subjectphytochemicals
dc.subjecttemperature
dc.titleProbing the Phytochemical Composition and Antioxidant Activity of Moringa oleifera under Ideal Germination Conditions
dc.typeJournal article
pubs.elements-id480236
pubs.organisational-groupOther
Files
Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
Published version.pdf
Size:
5.75 MB
Format:
Adobe Portable Document Format
Description:
480236 PDF.pdf
Download
Thumbnail Image
Name:
Evidence.pdf
Size:
164.65 KB
Format:
Adobe Portable Document Format
Description:
plants-2445170-supplementary.pdf
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
9.22 KB
Format:
Plain Text
Description:
Download
Collections
Journal Articles