A novel mutation in IAA16 is associated with dicamba resistance in Chenopodium album

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dc.citation.issue7
dc.citation.volume80
dc.contributor.authorGhanizadeh H
dc.contributor.authorHe L
dc.contributor.authorGriffiths AG
dc.contributor.authorHarrington KC
dc.contributor.authorCarbone V
dc.contributor.authorWu H
dc.contributor.authorTian K
dc.contributor.authorBo H
dc.contributor.authorXinhui D
dc.coverage.spatialEngland
dc.date.accessioned2024-07-28T23:21:41Z
dc.date.available2024-07-28T23:21:41Z
dc.date.issued2024-07
dc.description.abstractBACKGROUND: Resistance to dicamba in Chenopodium album was first documented over a decade ago, however, the molecular basis of dicamba resistance in this species has not been elucidated. In this research, the resistance mechanism in a dicamba-resistant C. album phenotype was investigated using a transcriptomics (RNA-sequence) approach. RESULTS: The dose-response assay showed that the resistant (R) phenotype was nearly 25-fold more resistant to dicamba than a susceptible (S) phenotype of C. album. Also, dicamba treatment significantly induced transcription of the known auxin-responsive genes, Gretchen Hagen 3 (GH3), small auxin-up RNAs (SAURs), and 1-aminocyclopropane-1-carboxylate synthase (ACS) genes in the susceptible phenotype. Comparing the transcripts of auxin TIR/AFB receptors and auxin/indole-3-acetic acid (AUX/IAA) proteins identified from C. album transcriptomic analysis revealed that the R phenotype contained a novel mutation at the first codon of the GWPPV degron motif of IAA16, resulting in an amino acid substitution of glycine (G) with aspartic acid (D). Sequencing the IAA16 gene in other R and S individuals further confirmed that all the R individuals contained the mutation. CONCLUSION: In this research, we describe the dicamba resistance mechanism in the only case of dicamba-resistant C. album reported to date. Prior work has shown that the dicamba resistance allele confers significant growth defects to the R phenotype investigated here, suggesting that dicamba-resistant C. album carrying this novel mutation in the IAA16 gene may not persist at high frequencies upon removal of dicamba application.
dc.description.confidentialfalse
dc.edition.editionJuly 2024
dc.format.pagination3675-3683
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/38459963
dc.identifier.citationGhanizadeh H, He L, Griffiths AG, Harrington KC, Carbone V, Wu H, Tian K, Bo H, Xinhui D. (2024). A novel mutation in IAA16 is associated with dicamba resistance in Chenopodium album.. Pest Manag Sci. 80. 7. (pp. 3675-3683).
dc.identifier.doi10.1002/ps.8071
dc.identifier.eissn1526-4998
dc.identifier.elements-typejournal-article
dc.identifier.issn1526-498X
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71123
dc.languageeng
dc.publisherJohn Wiley and Sons Ltd on behalf of Society of Chemical Industry
dc.publisher.urihttps://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.8071
dc.relation.isPartOfPest Manag Sci
dc.rights(c) 2024 The Author/s
dc.rightsCC BY-NC-ND 4.0
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAUX/IAA proteins
dc.subjectdicamba
dc.subjectherbicide resistance
dc.subjectmechanism of resistance
dc.subjectsynthetic auxin herbicides
dc.subjectChenopodium album
dc.subjectHerbicide Resistance
dc.subjectPlant Proteins
dc.subjectDicamba
dc.subjectMutation
dc.subjectHerbicides
dc.subjectIndoleacetic Acids
dc.titleA novel mutation in IAA16 is associated with dicamba resistance in Chenopodium album
dc.typeJournal article
pubs.elements-id487299
pubs.organisational-groupOther
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