Transcriptome and translational signaling following endurance exercise in trained skeletal muscle: Impact of dietary protein

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dc.citation.issue17
dc.citation.volume43
dc.contributor.authorRowlands DS
dc.contributor.authorThomson JS
dc.contributor.authorTimmons BW
dc.contributor.authorRaymond F
dc.contributor.authorFuerholz A
dc.contributor.authorMansourian R
dc.contributor.authorZwahlen M-C
dc.contributor.authorMétairon S
dc.contributor.authorGlover E
dc.contributor.authorStellingwerff T
dc.contributor.authorKussmann M
dc.contributor.authorTarnopolsky MA
dc.date.available1/09/2011
dc.date.issued2011
dc.description.abstractPostexercise protein feeding regulates the skeletal muscle adaptive response to endurance exercise, but the transcriptome guiding these adaptations in well-trained human skeletal muscle is uncharacterized. In a crossover design, eight cyclists ingested beverages containing protein, carbohydrate and fat (PTN: 0.4, 1.2, 0.2 g/kg, respectively) or isocaloric carbohydrate and fat (CON: 1.6, 0.2 g/kg) at 0 and 1 h following 100 min of cycling. Biopsies of the vastus lateralis were collected at 3 and 48 h following to determine the early and late transcriptome and regulatory signaling responses via microarray and immunoblot. The top gene ontology enriched by PTN were: muscle contraction, extracellular matrix--signaling and structure, and nucleoside, nucleotide, and nucleic acid metabolism (3 and 48 h); developmental processes, immunity, and defense (3 h); glycolysis, lipid and fatty acid metabolism (48 h). The transcriptome was also enriched within axonal guidance, actin cytoskeletal, Ca2+, cAMP, MAPK, and PPAR canonical pathways linking protein nutrition to exercise-stimulated signaling regulating extracellular matrix, slow-myofibril, and metabolic gene expression. At 3 h, PTN attenuated AMPKα1Thr172 phosphorylation but increased mTORC1Ser2448, rps6Ser240/244, and 4E-BP1-γ phosphorylation, suggesting increased translation initiation, while at 48 h AMPKα1Thr172 phosphorylation and PPARG and PPARGC1A expression increased, supporting the late metabolic transcriptome, relative to CON. To conclude, protein feeding following endurance exercise affects signaling associated with cell energy status and translation initiation and the transcriptome involved in skeletal muscle development, slow-myofibril remodeling, immunity and defense, and energy metabolism. Further research should determine the time course and posttranscriptional regulation of this transcriptome and the phenotype responding to chronic postexercise protein feeding.
dc.description.publication-statusPublished
dc.format.extent1004 - 1020 (17)
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000294730000003&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=c5bb3b2499afac691c2e3c1a83ef6fef
dc.identifier.citationPHYSIOLOGICAL GENOMICS, 2011, 43 (17), pp. 1004 - 1020 (17)
dc.identifier.doi10.1152/physiolgenomics.00073.2011
dc.identifier.elements-id94320
dc.identifier.harvestedMassey_Dark
dc.identifier.issn1094-8341
dc.identifier.urihttps://hdl.handle.net/10179/8073
dc.languageEnglish
dc.publisherAmerican Physiological Society
dc.relation.isPartOfPHYSIOLOGICAL GENOMICS
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectCell Biology
dc.subjectGenetics & Heredity
dc.subjectPhysiology
dc.subjectCELL BIOLOGY
dc.subjectGENETICS & HEREDITY
dc.subjectPHYSIOLOGY
dc.subjectnutrigenomics
dc.subjectmitochondrial biogenesis
dc.subjectfatty acid oxidation
dc.subjectextracellular matrix
dc.subjectAMP-activated protein kinase
dc.subjectGENE-EXPRESSION
dc.subjectMESSENGER-RNA
dc.subjectMITOCHONDRIAL BIOGENESIS
dc.subjectPGC-1-ALPHA PROMOTER
dc.subjectS6K1 PHOSPHORYLATION
dc.subjectDENDRITIC CELLS
dc.subjectAXON GUIDANCE
dc.subjectTIME-COURSE
dc.subjectRECOVERY
dc.subjectKINASE
dc.subject.anzsrc1116 Medical Physiology
dc.titleTranscriptome and translational signaling following endurance exercise in trained skeletal muscle: Impact of dietary protein
dc.typeJournal article
pubs.notesNot known
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Health
pubs.organisational-group/Massey University/College of Health/School of Sport, Exercise and Nutrition
pubs.organisational-group/Massey University/College of Sciences
pubs.organisational-group/Massey University/College of Sciences/School of Food and Advanced Technology
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