Cerebrospinal fluid Lyme multiplex assay results are not diagnostic in horses with neuroborreliosis

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dc.citation.issue2
dc.citation.volume32
dc.contributor.authorJohnson AL
dc.contributor.authorJohnstone LK
dc.contributor.authorStefanovski D
dc.coverage.spatialUnited States
dc.date.accessioned2024-10-18T00:52:04Z
dc.date.available2024-10-18T00:52:04Z
dc.date.issued2018-03
dc.description.abstractBACKGROUND: The accuracy of the Lyme multiplex assay for the diagnosis of neuroborreliosis in horses is unknown. HYPOTHESIS/OBJECTIVES: To describe Lyme multiplex results in horses with a postmortem diagnosis of neuroborreliosis. The hypothesis was that paired serum and cerebrospinal fluid (CSF) results and a CSF : serum ratio would allow differentiation of horses with neuroborreliosis from those with other neurologic diseases. ANIMALS: Ninety horses that had neurologic examinations, serum and CSF Lyme multiplex analyses, and postmortem examination of the nervous system performed. METHODS: Retrospective study. Data collected included signalment, ante- and postmortem diagnoses, and serum and CSF Lyme multiplex results. The CSF : serum ratio was calculated by dividing CSF median fluorescent intensity (MFI) by serum MFI for each result. RESULTS: Ten horses had a final diagnosis of neuroborreliosis, 70 were diagnosed with other neurologic diseases, and 10 had no neurologic disease. Not all horses with neuroborreliosis had positive results: 4/10 had at least 1 positive serum result, 5/10 had at least 1 positive CSF result, and 3/10 had at least 1 CSF result 4-fold higher than the corresponding serum result. Results were similar for the 70 horses with other neurologic diseases: 53% had at least 1 positive serum result, 50% had at least 1 positive CSF result, and 16% had at least 1 CSF result 4-fold higher than the corresponding serum result. CONCLUSIONS AND CLINICAL IMPORTANCE: Positive Lyme multiplex results were common in horses with neurologic diseases and did not adequately differentiate horses with neuroborreliosis from horses with other disorders.
dc.description.confidentialfalse
dc.edition.editionMarch/April 2018
dc.format.pagination832-838
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/29460492
dc.identifier.citationJohnson AL, Johnstone LK, Stefanovski D. (2018). Cerebrospinal fluid Lyme multiplex assay results are not diagnostic in horses with neuroborreliosis.. J Vet Intern Med. 32. 2. (pp. 832-838).
dc.identifier.doi10.1111/jvim.15067
dc.identifier.eissn1939-1676
dc.identifier.elements-typejournal-article
dc.identifier.issn0891-6640
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71757
dc.languageeng
dc.publisherWiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine.
dc.publisher.urihttps://onlinelibrary.wiley.com/doi/10.1111/jvim.15067
dc.relation.isPartOfJ Vet Intern Med
dc.rights(c) 2018 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBorrelia burgdorferi
dc.subjectborreliosis
dc.subjectimmunology
dc.subjectserology
dc.subjectAnimals
dc.subjectAntigens, Bacterial
dc.subjectBorrelia burgdorferi
dc.subjectHorse Diseases
dc.subjectHorses
dc.subjectImmunoassay
dc.subjectLyme Neuroborreliosis
dc.subjectNervous System Diseases
dc.subjectRetrospective Studies
dc.subjectSeroepidemiologic Studies
dc.titleCerebrospinal fluid Lyme multiplex assay results are not diagnostic in horses with neuroborreliosis
dc.typeJournal article
pubs.elements-id462400
pubs.organisational-groupOther
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