The impact of heating and drying on protease activities of ruminant milk before and after in vitro infant digestion

Leite JAS; Montoya CA; Loveday SM; Mullaney JA; Loo TS; McNabb WC; Roy NC

The impact of heating and drying on protease activities of ruminant milk before and after in vitro infant digestion

dc.citation.volume	429
dc.contributor.author	Leite JAS
dc.contributor.author	Montoya CA
dc.contributor.author	Loveday SM
dc.contributor.author	Mullaney JA
dc.contributor.author	Loo TS
dc.contributor.author	McNabb WC
dc.contributor.author	Roy NC
dc.coverage.spatial	England
dc.date.accessioned	2024-11-18T19:14:25Z
dc.date.available	2024-11-18T19:14:25Z
dc.date.issued	2023-12-15
dc.description.abstract	This study investigated the effect of heating (63°C/30 min or 75°C/15 s) and drying (spray-drying or freeze-drying) on plasmin, cathepsin D, and elastase activities in bovine, ovine, and caprine milk, compared to non-dried raw milk counterparts. Protease activities and protein hydrolysis were assessed before and after in vitro infant digestion with or without gastric and pancreatic enzymes. At 75°C/15 s, plasmin activity in caprine and ovine milk decreased (69-75%, p<0.05), while cathepsin D activity in spray-dried bovine milk heated increased (2.8-fold, p<0.05). Plasmin and cathepsin D activities increased (<1.2-fold, p<0.05) after in vitro digestion with pancreatin, regardless of milk species. Endogenous milk enzymes hydrolyzed more proteins than gastric enzymes during gastric digestion and contributed to small intestinal digestion. In summary, milk proteases remained active after processing with effects dependent on the species of milk, and they contributed to in vitro protein hydrolysis in the stomach and small intestine.
dc.description.confidential	false
dc.format.pagination	136979-
dc.identifier.author-url	https://www.ncbi.nlm.nih.gov/pubmed/37506658
dc.identifier.citation	Leite JAS, Montoya CA, Loveday SM, Mullaney JA, Loo TS, McNabb WC, Roy NC. (2023). The impact of heating and drying on protease activities of ruminant milk before and after in vitro infant digestion.. Food Chem. 429. (pp. 136979-).
dc.identifier.doi	10.1016/j.foodchem.2023.136979
dc.identifier.eissn	1873-7072
dc.identifier.elements-type	journal-article
dc.identifier.issn	0308-8146
dc.identifier.number	136979
dc.identifier.pii	S0308-8146(23)01597-2
dc.identifier.uri	https://mro.massey.ac.nz/handle/10179/72019
dc.language	eng
dc.publisher	Elsevier Ltd
dc.publisher.uri	https://www.sciencedirect.com/science/article/pii/S0308814623015972
dc.relation.isPartOf	Food Chem
dc.rights	(c) 2023 The Author/s
dc.rights	CC BY-NC-ND 4.0
dc.rights.uri	https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject	Digestion
dc.subject	Drying
dc.subject	Heating
dc.subject	Proteases
dc.subject	Protein hydrolysis
dc.subject	Ruminant milk
dc.subject	Humans
dc.subject	Infant
dc.subject	Animals
dc.subject	Sheep
dc.subject	Goats
dc.subject	Milk
dc.subject	Ruminants
dc.subject	Milk Proteins
dc.subject	Proteolysis
dc.subject	Digestion
dc.subject	Hot Temperature
dc.subject	Cathepsin D
dc.title	The impact of heating and drying on protease activities of ruminant milk before and after in vitro infant digestion
dc.type	Journal article
pubs.elements-id	479666
pubs.organisational-group	Other