Actinidin in Green and SunGold Kiwifruit Improves Digestion of Alternative Proteins-An In Vitro Investigation

Simple item page
dc.citation.issue18
dc.citation.volume11
dc.contributor.authorKaur L
dc.contributor.authorMao B
dc.contributor.authorBailly J
dc.contributor.authorOladeji O
dc.contributor.authorBlatchford P
dc.contributor.authorMcNabb WC
dc.contributor.editorRecio I
dc.coverage.spatialSwitzerland
dc.date.accessioned2023-10-20T02:14:49Z
dc.date.accessioned2023-10-25T20:49:36Z
dc.date.available2022-09-06
dc.date.available2023-10-20T02:14:49Z
dc.date.available2023-10-25T20:49:36Z
dc.date.issued2022-09-06
dc.date.updated2023-10-19T23:21:00Z
dc.description.abstractBoth Hayward (green) and SunGold (gold) kiwifruit varieties contain a proteolytic enzyme, actinidin, that has been reported to enhance the upper tract digestion of animal proteins. Unlike the other gold varieties, which do not contain any actinidin, the SunGold variety contains significantly higher actinidin activity, but its activity is still much lower than that present in the green (Hayward) fruit. The objective of this study was to determine the effectiveness of actinidin in Hayward and SunGold kiwifruit in digesting alternative proteins, including pea protein, almonds, tofu, and quinoa. The protein sources were digested using a three-stage in vitro oral-gastro-small intestinal digestion model. The findings showed that both kiwifruit extracts enhanced the breakdown (observed through SDS-PAGE) for all the studied protein sources, particularly during gastric digestion, possibly due to higher actinidin activity at gastric pH. The increase in the rate of protein breakdown was probably due to the broader specificity of actinidin compared to pepsin. For many protein sources, most of the intact proteins disappeared within the first few minutes of gastric digestion with added kiwifruit extract. Green kiwifruit extract, due to its higher actinidin activity, had a higher effect on protein breakdown than the SunGold extract. However, for some proteins and under certain digestion conditions, SunGold extract resulted in higher protein breakdown. The latter, in the absence of any digestive enzymes, also led to some protein breakdown during the small intestinal digestion phase, which was not the case for the green kiwifruit extract. The green kiwifruit extract led to the greater breakdown of polypeptide chains of Pru-du 6, a major allergen in almonds. The results, for the first time, suggest that both Hayward and SunGold kiwifruit can lead to improved breakdown and digestion of alternative proteins when consumed as part of a meal; and therefore, have the potential to be used as a digestive aid in population groups looking to achieve faster and greater protein digestion such as athletes, elderly and people with the impaired digestive system.
dc.description.confidentialfalse
dc.edition.editionSeptember 2022
dc.format.extent2739-
dc.identifier2739
dc.identifierfoods11182739
dc.identifierhttps://www.ncbi.nlm.nih.gov/pubmed/36140865
dc.identifier.citationKaur L, Mao B, Bailly J, Oladeji O, Blatchford P, McNabb WC. (2022). Actinidin in Green and SunGold Kiwifruit Improves Digestion of Alternative Proteins-An In Vitro Investigation.. Foods. 11. 18. (pp. 2739-).
dc.identifier.doi10.3390/foods11182739
dc.identifier.eissn2304-8158
dc.identifier.elements-typejournal-article
dc.identifier.harvestedMassey_Dark
dc.identifier.issn2304-8158
dc.identifier.urihttp://hdl.handle.net/10179/20397
dc.languageeng
dc.publisherMDPI (Basel, Switzerland)
dc.publisher.urihttps://www.mdpi.com/2304-8158/11/18/2739
dc.relation.isPartOfFoods
dc.rights(c) 2022 The Author/sen_US
dc.rightsCC BYen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subjectSDS-PAGE
dc.subjectactinidin
dc.subjectalternative proteins
dc.subjectgold kiwifruit
dc.subjectgreen kiwifruit
dc.subjectin vitro digestion
dc.titleActinidin in Green and SunGold Kiwifruit Improves Digestion of Alternative Proteins-An In Vitro Investigation
dc.typeJournal article
pubs.elements-id457171
pubs.organisational-groupOther
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
457171 PDF.pdf
Size:
8.59 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Journal Articles