General Anaesthesia Shifts the Murine Circadian Clock in a Time-Dependant Fashion

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dc.citation.issue1
dc.citation.volume3
dc.contributor.authorLudin NM
dc.contributor.authorOrts-Sebastian A
dc.contributor.authorCheeseman JF
dc.contributor.authorChong J
dc.contributor.authorMerry AF
dc.contributor.authorCumin D
dc.contributor.authorYamazaki S
dc.contributor.authorPawley MDM
dc.contributor.authorWarman GR
dc.coverage.spatialSwitzerland
dc.date.accessioned2024-01-07T22:16:55Z
dc.date.accessioned2024-07-25T06:49:26Z
dc.date.available2021-01-26
dc.date.available2024-01-07T22:16:55Z
dc.date.available2024-07-25T06:49:26Z
dc.date.issued2021-01-26
dc.description.abstractFollowing general anaesthesia (GA), patients frequently experience sleep disruption and fatigue, which has been hypothesized to result at least in part by GA affecting the circadian clock. Here, we provide the first comprehensive time-dependent analysis of the effects of the commonly administered inhalational anaesthetic, isoflurane, on the murine circadian clock, by analysing its effects on (a) behavioural locomotor rhythms and (b) PER2::LUC expression in the suprachiasmatic nuclei (SCN) of the mouse brain. Behavioural phase shifts elicited by exposure of mice (n = 80) to six hours of GA (2% isoflurane) were determined by recording wheel-running rhythms in constant conditions (DD). Phase shifts in PER2::LUC expression were determined by recording bioluminescence in organotypic SCN slices (n = 38) prior to and following GA exposure (2% isoflurane). Full phase response curves for the effects of GA on behaviour and PER2::LUC rhythms were constructed, which show that the effects of GA are highly time-dependent. Shifts in SCN PER2 expression were much larger than those of behaviour (c. 0.7 h behaviour vs. 7.5 h PER2::LUC). We discuss the implications of this work for understanding how GA affects the clock, and how it may inform the development of chronotherapeutic strategies to reduce GA-induced phase-shifting in patients.
dc.description.confidentialfalse
dc.edition.editionMarch 2021
dc.format.pagination87-97
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/33530488
dc.identifier.citationLudin NM, Orts-Sebastian A, Cheeseman JF, Chong J, Merry AF, Cumin D, Yamazaki S, Pawley MDM, Warman GR. (2021). General Anaesthesia Shifts the Murine Circadian Clock in a Time-Dependant Fashion.. Clocks Sleep. 3. 1. (pp. 87-97).
dc.identifier.doi10.3390/clockssleep3010006
dc.identifier.eissn2624-5175
dc.identifier.elements-typejournal-article
dc.identifier.issn2624-5175
dc.identifier.piiclockssleep3010006
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/70957
dc.languageeng
dc.publisherMDPI (Basel, Switzerland)
dc.publisher.urihttps://www.mdpi.com/2624-5175/3/1/6
dc.relation.isPartOfClocks Sleep
dc.rights(c) 2021 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectPERIOD2::LUC
dc.subjectcircadian clock
dc.subjectclock gene
dc.subjectgeneral anaesthesia
dc.subjectphase response curve
dc.titleGeneral Anaesthesia Shifts the Murine Circadian Clock in a Time-Dependant Fashion
dc.typeJournal article
pubs.elements-id440122
pubs.organisational-groupOther
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