Genome Analysis and Therapeutic Evaluation of a Novel Lytic Bacteriophage of Salmonella Typhimurium: Suggestive of a New Genus in the Subfamily Vequintavirinae.

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dc.citation.issue2
dc.citation.volume14
dc.contributor.authorSattar S
dc.contributor.authorUllah I
dc.contributor.authorKhanum S
dc.contributor.authorBailie M
dc.contributor.authorShamsi B
dc.contributor.authorAhmed I
dc.contributor.authorAbbas Shah T
dc.contributor.authorJaved S
dc.contributor.authorGhafoor A
dc.contributor.authorPervaiz A
dc.contributor.authorSohail F
dc.contributor.authorImdad K
dc.contributor.authorTariq A
dc.contributor.authorBostan N
dc.contributor.authorAli I
dc.contributor.authorAltermann E
dc.contributor.editorGriffiths M
dc.contributor.editorAnany H
dc.coverage.spatialSwitzerland
dc.date.accessioned2024-08-15T01:28:15Z
dc.date.available2024-08-15T01:28:15Z
dc.date.issued2022-01-25
dc.description.abstractSalmonella Typhimurium, a foodborne pathogen, is a major concern for food safety. Its MDR serovars of animal origin pose a serious threat to the human population. Phage therapy can be an alternative for the treatment of such MDR Salmonella serovars. In this study, we report on detailed genome analyses of a novel Salmonella phage (Salmonella-Phage-SSBI34) and evaluate its therapeutic potential. The phage was evaluated for latent time, burst size, host range, and bacterial growth reduction in liquid cultures. The phage stability was examined at various pH levels and temperatures. The genome analysis (141.095 Kb) indicated that its nucleotide sequence is novel, as it exhibited only 1-7% DNA coverage. The phage genome features 44% GC content, and 234 putative open reading frames were predicted. The genome was predicted to encode for 28 structural proteins and 40 enzymes related to nucleotide metabolism, DNA modification, and protein synthesis. Further, the genome features 11 tRNA genes for 10 different amino acids, indicating alternate codon usage, and hosts a unique hydrolase for bacterial lysis. This study provides new insights into the subfamily Vequintavirinae, of which SSBI34 may represent a new genus.
dc.description.confidentialfalse
dc.edition.edition2022
dc.format.pagination241-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/35215834
dc.identifier.citationSattar S, Ullah I, Khanum S, Bailie M, Shamsi B, Ahmed I, Abbas Shah T, Javed S, Ghafoor A, Pervaiz A, Sohail F, Imdad K, Tariq A, Bostan N, Ali I, Altermann E. (2022). Genome Analysis and Therapeutic Evaluation of a Novel Lytic Bacteriophage of Salmonella Typhimurium: Suggestive of a New Genus in the Subfamily Vequintavirinae.. Viruses. 14. 2. (pp. 241-).
dc.identifier.doi10.3390/v14020241
dc.identifier.eissn1999-4915
dc.identifier.elements-typejournal-article
dc.identifier.issn1999-4915
dc.identifier.number241
dc.identifier.piiv14020241
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71310
dc.languageeng
dc.publisherMDPI (Basel, Switzerland)
dc.publisher.urihttps://www.mdpi.com/1999-4915/14/2/241
dc.relation.isPartOfViruses
dc.rights(c) 2022 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectSalmonella Typhimurium
dc.subjectSalmonella-specific bacteriophage
dc.subjectVequintavirinae
dc.subjectgenome analysis
dc.subjectlytic phages
dc.subjectAnimals
dc.subjectBacteriolysis
dc.subjectBiological Control Agents
dc.subjectGenome, Viral
dc.subjectHost Specificity
dc.subjectMyoviridae
dc.subjectOpen Reading Frames
dc.subjectPhage Therapy
dc.subjectPhylogeny
dc.subjectPoultry
dc.subjectSalmonella Infections
dc.subjectSalmonella Phages
dc.subjectSalmonella typhimurium
dc.titleGenome Analysis and Therapeutic Evaluation of a Novel Lytic Bacteriophage of Salmonella Typhimurium: Suggestive of a New Genus in the Subfamily Vequintavirinae.
dc.typeJournal article
pubs.elements-id486130
pubs.organisational-groupOther
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