Standardising the lactulose mannitol test of gut permeability to minimise error and promote comparability.

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dc.citation.issue6
dc.citation.volume9
dc.contributor.authorSequeira IR
dc.contributor.authorLentle RG
dc.contributor.authorKruger MC
dc.contributor.authorHurst RD
dc.date.available2014-06-05
dc.date.available2014-05-13
dc.date.issued2014
dc.description© 2014 Sequeira et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.description.abstractBACKGROUND: Lactulose mannitol ratio tests are clinically useful for assessing disorders characterised by changes in gut permeability and for assessing mixing in the intestinal lumen. Variations between currently used test protocols preclude meaningful comparisons between studies. We determined the optimal sampling period and related this to intestinal residence. METHODS: Half-hourly lactulose and mannitol urinary excretions were determined over 6 hours in 40 healthy female volunteers after administration of either 600 mg aspirin or placebo, in randomised order at weekly intervals. Gastric and small intestinal transit times were assessed by the SmartPill in 6 subjects from the same population. Half-hourly percentage recoveries of lactulose and mannitol were grouped on a basis of compartment transit time. The rate of increase or decrease of each sugar within each group was explored by simple linear regression to assess the optimal period of sampling. KEY RESULTS: The between subject standard errors for each half-hourly lactulose and mannitol excretion were lowest, the correlation of the quantity of each sugar excreted with time was optimal and the difference between the two sugars in this temporal relationship maximal during the period from 2½-4 h after ingestion. Half-hourly lactulose excretions were generally increased after dosage with aspirin whilst those of mannitol were unchanged as was the temporal pattern and period of lowest between subject standard error for both sugars. CONCLUSION: The results indicate that between subject variation in the percentage excretion of the two sugars would be minimised and the differences in the temporal patterns of excretion would be maximised if the period of collection of urine used in clinical tests of small intestinal permeability were restricted to 2½-4 h post dosage. This period corresponds to a period when the column of digesta column containing the probes is passing from the small to the large intestine.
dc.description.publication-statusPublished
dc.format.extent? - ? (11)
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000336841400114&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=c5bb3b2499afac691c2e3c1a83ef6fef
dc.identifierARTN e99256
dc.identifier.citationPLOS ONE, 2014, 9 (6), pp. ? - ? (11)
dc.identifier.doi10.1371/journal.pone.0099256
dc.identifier.elements-id212273
dc.identifier.issn1932-6203
dc.languageEnglish
dc.publisherPUBLIC LIBRARY SCIENCE
dc.relation.isPartOfPLOS ONE
dc.rights2014 Sequeira et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.subjectScience & Technology
dc.subjectMultidisciplinary Sciences
dc.subjectScience & Technology - Other Topics
dc.subjectMULTIDISCIPLINARY SCIENCES
dc.subjectINTESTINAL PERMEABILITY
dc.subjectCROHNS-DISEASE
dc.subjectCELIAC-DISEASE
dc.subjectGASTROINTESTINAL TRANSIT
dc.subjectGLUCOSE-TOLERANCE
dc.subjectINSULIN-SECRETION
dc.subjectSUGAR ABSORPTION
dc.subjectDEGREE RELATIVES
dc.subjectCAPSULE
dc.subjectLENGTH
dc.titleStandardising the lactulose mannitol test of gut permeability to minimise error and promote comparability.
dc.typeJournal article
pubs.notesNot known
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Health
pubs.organisational-group/Massey University/College of Health/School of Health Science
pubs.organisational-group/Massey University/College of Sciences
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