Polyploidy on islands - concerted evolution and gene loss amid chromosomal stasis

Simple item page
dc.citation.issue1
dc.citation.volume131
dc.contributor.authorJoshi P
dc.contributor.authorAnsari H
dc.contributor.authorDickson R
dc.contributor.authorEllison NW
dc.contributor.authorSkema C
dc.contributor.authorTate JA
dc.coverage.spatialEngland
dc.date.accessioned2024-01-11T22:20:55Z
dc.date.accessioned2024-07-25T06:47:06Z
dc.date.available2022-04-07
dc.date.available2024-01-11T22:20:55Z
dc.date.available2024-07-25T06:47:06Z
dc.date.issued2023-01-01
dc.description.abstractBACKGROUND AND AIMS: Polyploidy is an important process that often generates genomic diversity within lineages, but it can also cause changes that result in loss of genomic material. Island lineages, while often polyploid, typically show chromosomal stasis but have not been investigated in detail regarding smaller-scale gene loss. Our aim was to investigate post-polyploidization genome dynamics in a chromosomally stable lineage of Malvaceae endemic to New Zealand. METHODS: We determined chromosome numbers and used fluorescence in situ hybridization to localize 18S and 5S rDNA. Gene sequencing of 18S rDNA, the internal transcribed spacers (ITS) with intervening 5.8S rDNA, and a low-copy nuclear gene, GBSSI-1, was undertaken to determine if gene loss occurred in the New Zealand lineage following polyploidy. KEY RESULTS: The chromosome number for all species investigated was 2nā€…=ā€…42, with the first published report for the monotypic Australian genus Asterotrichion. The five species investigated all had two 5S rDNA signals localized interstitially on the long arm of one of the largest chromosome pairs. All species, except Plagianthus regius, had two 18S rDNA signals localized proximally on the short arm of one of the smallest chromosome pairs. Plagianthus regius had two additional 18S rDNA signals on a separate chromosome, giving a total of four. Sequencing of nuclear ribosomal 18S rDNA and the ITS cistron indicated loss of historical ribosomal repeats. Phylogenetic analysis of a low-copy nuclear gene, GBSSI-1, indicated that some lineages maintained three copies of the locus, while others have lost one or two copies. CONCLUSIONS: Although island endemic lineages show chromosomal stasis, with no additional changes in chromosome number, they may undergo smaller-scale processes of gene loss and concerted evolution ultimately leading to further genome restructuring and downsizing.
dc.description.confidentialfalse
dc.format.pagination33-44
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/35390127
dc.identifier.citationJoshi P, Ansari H, Dickson R, Ellison NW, Skema C, Tate JA. (2023). Polyploidy on islands - concerted evolution and gene loss amid chromosomal stasis.. Ann Bot. 131. 1. (pp. 33-44).
dc.identifier.doi10.1093/aob/mcac051
dc.identifier.eissn1095-8290
dc.identifier.elements-typejournal-article
dc.identifier.issn0305-7364
dc.identifier.pii6564895
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/70866
dc.languageeng
dc.publisherOxford University Press on behalf of the Annals of Botany Company
dc.publisher.urihttps://academic.oup.com/aob/article/131/1/33/6564895
dc.relation.isPartOfAnn Bot
dc.rights(c) 2022 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectPlagianthus alliance
dc.subject18S rDNA
dc.subjectGBSSI
dc.subjectITS
dc.subjectMalvaceae
dc.subjectchromosome number
dc.subjectconcerted evolution
dc.subjectfluorescence in situ hybridization
dc.subjectgene fractionation
dc.subjectpolyploid
dc.subjectrDNA
dc.subjectPhylogeny
dc.subjectIn Situ Hybridization, Fluorescence
dc.subjectAustralia
dc.subjectChromosomes
dc.subjectDNA, Ribosomal
dc.subjectPolyploidy
dc.titlePolyploidy on islands - concerted evolution and gene loss amid chromosomal stasis
dc.typeJournal article
pubs.elements-id452830
pubs.organisational-groupOther
Files
Original bundle
Now showing 1 - 3 of 3
Thumbnail Image
Name:
Evidence 2
Size:
329.99 KB
Format:
Microsoft Word XML
Description:
Download
Thumbnail Image
Name:
Published version
Size:
1.61 MB
Format:
Adobe Portable Document Format
Description:
Download
Thumbnail Image
Name:
Evidence 1
Size:
329.99 KB
Format:
Microsoft Word XML
Description:
Download
Collections
Journal Articles