Characterization of two novel lytic bacteriophages having lysis potential against MDR avian pathogenic Escherichia coli strains of zoonotic potential.

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dc.citation.issue1
dc.citation.volume13
dc.contributor.authorSattar S
dc.contributor.authorBailie M
dc.contributor.authorYaqoob A
dc.contributor.authorKhanum S
dc.contributor.authorFatima K
dc.contributor.authorAltaf AURB
dc.contributor.authorAhmed I
dc.contributor.authorShah STA
dc.contributor.authorMunawar J
dc.contributor.authorZehra QA
dc.contributor.authorDaud S
dc.contributor.authorArshad A
dc.contributor.authorImdad K
dc.contributor.authorJaved S
dc.contributor.authorTariq A
dc.contributor.authorBostan N
dc.contributor.authorAltermann E
dc.coverage.spatialEngland
dc.date.accessioned2024-08-19T02:39:43Z
dc.date.available2024-08-19T02:39:43Z
dc.date.issued2023-06-20
dc.description.abstractAvian pathogenic E. coli (APEC) is associated with local and systemic infections in poultry, ducks, turkeys, and many other avian species, leading to heavy economical losses. These APEC strains are presumed to possess zoonotic potential due to common virulence markers that can cause urinary tract infections in humans. The prophylactic use of antibiotics in the poultry sector has led to the rapid emergence of Multiple Drug Resistant (MDR) APEC strains that act as reservoirs and put human populations at risk. This calls for consideration of alternative strategies to decrease the bacterial load. Here, we report isolation, preliminary characterization, and genome analysis of two novel lytic phage species (Escherichia phage SKA49 and Escherichia phage SKA64) against MDR strain of APEC, QZJM25. Both phages were able to keep QZJM25 growth significantly less than the untreated bacterial control for approximately 18 h. The host range was tested against Escherichia coli strains of poultry and human UTI infections. SKA49 had a broader host range in contrast to SKA64. Both phages were stable at 37 °C only. Their genome analysis indicated their safety as no recombination, integration and host virulence genes were identified. Both these phages can be good candidates for control of APEC strains based on their lysis potential.
dc.description.confidentialfalse
dc.edition.edition2023
dc.format.pagination10043-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/37340022
dc.identifier.citationSattar S, Bailie M, Yaqoob A, Khanum S, Fatima K, Altaf AURB, Ahmed I, Shah STA, Munawar J, Zehra QA, Daud S, Arshad A, Imdad K, Javed S, Tariq A, Bostan N, Altermann E. (2023). Characterization of two novel lytic bacteriophages having lysis potential against MDR avian pathogenic Escherichia coli strains of zoonotic potential.. Sci Rep. 13. 1. (pp. 10043-).
dc.identifier.doi10.1038/s41598-023-37176-z
dc.identifier.eissn2045-2322
dc.identifier.elements-typejournal-article
dc.identifier.issn2045-2322
dc.identifier.number10043
dc.identifier.pii10.1038/s41598-023-37176-z
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71337
dc.languageeng
dc.publisherSpringer Nature Limited
dc.publisher.urihttps://www.nature.com/articles/s41598-023-37176-z
dc.relation.isPartOfSci Rep
dc.rights(c) 2023 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAnimals
dc.subjectHumans
dc.subjectEscherichia coli
dc.subjectEscherichia coli Infections
dc.subjectBacteriophages
dc.subjectBirds
dc.subjectPoultry
dc.subjectPoultry Diseases
dc.subjectChickens
dc.titleCharacterization of two novel lytic bacteriophages having lysis potential against MDR avian pathogenic Escherichia coli strains of zoonotic potential.
dc.typeJournal article
pubs.elements-id486123
pubs.organisational-groupOther
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