An Improved Purification Method for Removing Colour Interference from 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl Tetrazolium Bromide (MTT) Antibacterial Assays

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dc.citation.issue8
dc.citation.volume13
dc.contributor.authorShi D
dc.contributor.authorXu W
dc.contributor.authorWong M
dc.contributor.authorPopovich DG
dc.contributor.editorKowalczewski PL
dc.contributor.editorKačániová M
dc.date.accessioned2024-11-28T01:39:40Z
dc.date.available2024-11-28T01:39:40Z
dc.date.issued2023-04-18
dc.description.abstractThe MTT antibacterial assay is an important method in natural antibacterial component discovery. Researchers can use the MTT antibacterial assay to quickly and efficiently evaluate the antibacterial activity of natural compounds. The aim of this research was to find a method to reduce background colour and bacterial cell interference when using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay to study the antibacterial effect of phytochemicals. This study used NZ ‘Hass’ ripe avocado seed extract as an example to develop a silica gel column chromatography method that could combine with the MTT assay to remove the background colour and bacterial cells before the formazan measuring stage. This method is particularly suitable for MTT-based antibacterial inhibition studies when the tested phytochemical extracts have an interfering colour.
dc.description.confidentialfalse
dc.edition.editionApril-2 2023
dc.identifier.citationShi D, Xu W, Wong M, Popovich DG. (2023). An Improved Purification Method for Removing Colour Interference from 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl Tetrazolium Bromide (MTT) Antibacterial Assays. Applied Sciences (Switzerland). 13. 8.
dc.identifier.doi10.3390/app13085067
dc.identifier.eissn2076-3417
dc.identifier.elements-typejournal-article
dc.identifier.number5067
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/72116
dc.languageEnglish
dc.publisherMDPI (Basel, Switzerland)
dc.publisher.urihttp://mdpi.com/2076-3417/13/8/5067
dc.relation.isPartOfApplied Sciences (Switzerland)
dc.rights(c) 2023 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectbackground colour
dc.subjectbacterial cell
dc.subjectMTT
dc.subjectsilica gel column chromatography method
dc.subjectformazan
dc.titleAn Improved Purification Method for Removing Colour Interference from 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl Tetrazolium Bromide (MTT) Antibacterial Assays
dc.typeJournal article
pubs.elements-id461444
pubs.organisational-groupCollege of Health
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