Monomorphic genotypes within a generalist lineage of Campylobacter jejuni show signs of global dispersion

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dc.citation.issue10
dc.citation.volume2
dc.contributor.authorLlarena AK
dc.contributor.authorZhang J
dc.contributor.authorVehkala M
dc.contributor.authorVälimäki N
dc.contributor.authorHakkinen M
dc.contributor.authorHänninen M
dc.contributor.authorRoasto M
dc.contributor.authorMäesaar M
dc.contributor.authorTaboada E
dc.contributor.authorBarker D
dc.contributor.authorGarofolo G
dc.contributor.authorCammà C
dc.contributor.authorDi Giannatale E
dc.contributor.authorCorander J
dc.contributor.authorRoss M
dc.date.available2016-10
dc.date.available2016-09-12
dc.date.issued2016-10-01
dc.description.abstractThe decreased costs of genome sequencing have increased the capability to apply whole-genome sequencing to epidemiological surveillance of zoonotic Campylobacter jejuni. However, knowledge of the genetic diversity of this bacteria is vital for inferring relatedness between epidemiologically linked isolates and a necessary prerequisite for correct application of this methodology. To address this issue in C. jejuni we investigated the spatial and temporal signals in the genomes of a major clonal complex and generalist lineage, ST-45 CC, by analysing the population structure and genealogy as well as applying genome-wide association analysis of 340 isolates from across Europe collected over a wide time range. The occurrence and strength of the geographical signal varied between sublineages and followed the clonal frame when present, while no evidence of a temporal signal was found. Certain sublineages of ST-45 formed discrete and genetically isolated clades containing isolates with extremely similar genomes regardless of time and location of sampling. Based on a separate data set, these monomorphic genotypes represent successful C. jejuni clones, possibly spread around the globe by rapid animal (migrating birds), food or human movement. In addition, we observed an incongruence between the genealogy of the strains and multilocus sequence typing (MLST), challenging the existing clonal complex definition and the use of whole-genome gene-by-gene hierarchical nomenclature schemes for C. jejuni.
dc.description.publication-statusPublished
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000431154200003&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=c5bb3b2499afac691c2e3c1a83ef6fef
dc.identifierARTN 000088
dc.identifier.citationMICROBIAL GENOMICS, 2016, 2 (10)
dc.identifier.doi10.1099/mgen.0.000088
dc.identifier.elements-id342392
dc.identifier.harvestedMassey_Dark
dc.identifier.issn2057-5858
dc.publisherMicrobiology Society
dc.relation.isPartOfMICROBIAL GENOMICS
dc.rights2016 The Authors. Published by Microbiology Society This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/)
dc.subjectCampylobacter jejuni
dc.subjectgenomic epidemiology
dc.subjectmonomorphic clones
dc.subjectwhole-genome sequencing
dc.subject.anzsrc0604 Genetics
dc.subject.anzsrc0605 Microbiology
dc.titleMonomorphic genotypes within a generalist lineage of Campylobacter jejuni show signs of global dispersion
dc.typeJournal article
pubs.notesNot known
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Sciences
pubs.organisational-group/Massey University/College of Sciences/School of Veterinary Science
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