Unambiguous identification of Ancylostoma caninum and Uncinaria stenocephala in Australian and New Zealand dogs from faecal samples.

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dc.citation.issue10
dc.citation.volume101
dc.contributor.authorStocker T
dc.contributor.authorScott I
dc.contributor.authorŠlapeta J
dc.coverage.spatialEngland
dc.date.accessioned2024-08-26T03:22:16Z
dc.date.available2024-08-26T03:22:16Z
dc.date.issued2023-10-01
dc.description.abstractHookworms (Ancylostomatidae) are well-known parasites in dogs due to their health impacts and zoonotic potential. While faecal analysis is the traditional method for detection, improvements in husbandry and deworming have decreased their prevalence in urban owned dogs. Drug resistance in Ancylostoma caninum is becoming a discussion point in small animal practices across the region. This study aimed to identify hookworm species present in Australian and New Zealand dogs using molecular techniques. The ITS-2 and isotype-1 β-tubulin assays were used to identify and quantify hookworm species. Results showed absence of coinfection in Australian samples from Greater Sydney region belonging either to A. caninum or Uncinaria stenocephala, while New Zealand samples were a mixture of A. caninum and U. stenocephala. The amplified isotype-1 β-tubulin sequences exhibited susceptibility to benzimidazole drugs. Rare mutations were identified in A. caninum and U. stenocephala sequences, representing a small percentage of reads. This study highlights the importance of molecular techniques in accurately identifying and quantifying hookworm species in dog populations.
dc.description.confidentialfalse
dc.edition.editionOctober 2023
dc.format.pagination373-376
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/37537874
dc.identifier.citationStocker T, Scott I, Šlapeta J. (2023). Unambiguous identification of Ancylostoma caninum and Uncinaria stenocephala in Australian and New Zealand dogs from faecal samples.. Aust Vet J. 101. 10. (pp. 373-376).
dc.identifier.doi10.1111/avj.13272
dc.identifier.eissn1751-0813
dc.identifier.elements-typejournal-article
dc.identifier.issn0005-0423
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71383
dc.languageeng
dc.publisherJohn Wiley and Sons, Inc.
dc.publisher.urihttps://onlinelibrary.wiley.com/doi/10.1111/avj.13272
dc.relation.isPartOfAust Vet J
dc.rights(c) 2023 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectITS-2
dc.subjectdrug resistance
dc.subjecthookworms
dc.subjectnemabiome
dc.subjecttubulin
dc.subjectDogs
dc.subjectAnimals
dc.subjectAncylostoma
dc.subjectAncylostomatoidea
dc.subjectNew Zealand
dc.subjectTubulin
dc.subjectDog Diseases
dc.subjectAustralia
dc.subjectHookworm Infections
dc.subjectFeces
dc.titleUnambiguous identification of Ancylostoma caninum and Uncinaria stenocephala in Australian and New Zealand dogs from faecal samples.
dc.typeJournal article
pubs.elements-id479676
pubs.organisational-groupOther
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