Characterization of the extracellular polymeric substances matrix of Pseudomonas biofilms formed at the air-liquid interface

Muthuraman S; Flint S; Palmer J

Characterization of the extracellular polymeric substances matrix of Pseudomonas biofilms formed at the air-liquid interface

dc.citation.issue	February 2025
dc.citation.volume	64
dc.contributor.author	Muthuraman S
dc.contributor.author	Flint S
dc.contributor.author	Palmer J
dc.date.accessioned	2025-03-07T01:08:55Z
dc.date.available	2025-03-07T01:08:55Z
dc.date.issued	2025-01-27
dc.description.abstract	Pseudomonas are common psychotropic food spoilage organisms that affect the quality of aerobically chilled food products. Biofilm formation of these bacteria on food contact surfaces can provide a continuous contamination source, leading to food spoilage. Pseudomonas produce proteolytic and lipolytic enzymes which lead to organoleptic degradation of stored food products. The biofilm extracellular polymeric substances matrix (EPS) protects the bacterial cells from CIP (Cleaning-In-Place) chemicals and adverse conditions. Studies on the composition of the EPS matrix and the molecules present in the EPS matrix are limited. In this study, the EPS composition of mono-species biofilms of Pseudomonas lundensis and Pseudomonas cedrina on polystyrene and stainless-steel surfaces was characterized by chemical analysis and microscopical observations. The biofilms were allowed to grow on polystyrene and stainless-steel surfaces with half-strength TSB for 2 weeks at 30 °C and cold chain temperatures of 7 °C and 4 °C. The EPS was extracted by sonication and centrifugation and chemically analysed for cellulose, total polysaccharides, total proteins, and eDNA. Pseudomonas isolates in this study formed biofilms at the air-liquid interface. The formation of ring-like structures of cells was observed on the polystyrene surface. eDNA formed as a thread-like structure on a polystyrene surface while it formed channels on a stainless-steel surface. The amount of EPS varied at different temperatures. More EPS was formed at 4 °C than 30 °C. Flagellin, Clp protease, Arginine deiminase, and ATP-Binding Cassette (ABC) transporter substrate-binding proteins were the key proteins identified in the biofilm matrix of P. lundensis.
dc.description.confidential	false
dc.identifier.citation	Muthuraman S, Flint S, Palmer J. (2025). Characterization of the extracellular polymeric substances matrix of Pseudomonas biofilms formed at the air-liquid interface. Food Bioscience. 64. February 2025.
dc.identifier.doi	10.1016/j.fbio.2025.105918
dc.identifier.eissn	2212-4306
dc.identifier.elements-type	journal-article
dc.identifier.issn	2212-4292
dc.identifier.number	105918
dc.identifier.pii	S221242922500094X
dc.identifier.uri	https://mro.massey.ac.nz/handle/10179/72596
dc.language	English
dc.publisher	Elsevier Ltd
dc.publisher.uri	https://www.sciencedirect.com/science/article/pii/S221242922500094X
dc.relation.isPartOf	Food Bioscience
dc.rights	(c) 2025 The Author/s
dc.rights	CC BY 4.0
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	Psychrotrophic
dc.subject	eDNA
dc.subject	Stainless-steel
dc.subject	Enzymes
dc.subject	Polysaccharides
dc.title	Characterization of the extracellular polymeric substances matrix of Pseudomonas biofilms formed at the air-liquid interface
dc.type	Journal article
pubs.elements-id	499578
pubs.organisational-group	Other