Competition for Hydrogen Prevents Coexistence of Human Gastrointestinal Hydrogenotrophs in Continuous Culture.

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dc.citation.volume11
dc.contributor.authorSmith NW
dc.contributor.authorShorten PR
dc.contributor.authorAltermann E
dc.contributor.authorRoy NC
dc.contributor.authorMcNabb WC
dc.contributor.editorKappler U
dc.coverage.spatialSwitzerland
dc.date.accessioned2024-08-07T23:06:30Z
dc.date.available2024-08-07T23:06:30Z
dc.date.issued2020-05-29
dc.description.abstractUnderstanding the metabolic dynamics of the human gastrointestinal tract (GIT) microbiota is of growing importance as research continues to link the microbiome to host health status. Microbial strains that metabolize hydrogen have been associated with a variety of both positive and negative host nutritional and health outcomes, but limited data exists for their competition in the GIT. To enable greater insight into the behaviour of these microbes, a mathematical model was developed for the metabolism and growth of the three major hydrogenotrophic groups: sulphate-reducing bacteria (SRB), methanogens and reductive acetogens. In batch culture simulations with abundant sulphate and hydrogen, the SRB outcompeted the methanogen for hydrogen due to having a half-saturation constant 106 times lower than that of the methanogen. The acetogen, with a high model threshold for hydrogen uptake of around 70 mM, was the least competitive. Under high lactate and zero sulphate conditions, hydrogen exchange between the SRB and the methanogen was the dominant interaction. The methanogen grew at 70% the rate of the SRB, with negligible acetogen growth. In continuous culture simulations, both the SRB and the methanogen were washed out at dilution rates above 0.15 h-1 regardless of substrate availability, whereas the acetogen could survive under abundant hydrogen conditions. Specific combinations of conditions were required for survival of more than one hydrogenotroph in continuous culture, and survival of all three was not possible. The stringency of these requirements and the inability of the model to simulate survival of all three hydrogenotrophs in continuous culture demonstrates that factors outside of those modelled are vital to allow hydrogenotroph coexistence in the GIT.
dc.description.confidentialfalse
dc.edition.edition2020
dc.format.pagination1073-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/32547517
dc.identifier.citationSmith NW, Shorten PR, Altermann E, Roy NC, McNabb WC. (2020). Competition for Hydrogen Prevents Coexistence of Human Gastrointestinal Hydrogenotrophs in Continuous Culture.. Front Microbiol. 11. (pp. 1073-).
dc.identifier.doi10.3389/fmicb.2020.01073
dc.identifier.eissn1664-302X
dc.identifier.elements-typejournal-article
dc.identifier.issn1664-302X
dc.identifier.numberARTN 1073
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71231
dc.languageeng
dc.publisherFrontiers Media S.A.
dc.publisher.urihttps://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.01073/full
dc.relation.isPartOfFront Microbiol
dc.rights(c) 2020 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectacetate
dc.subjectcross-feeding
dc.subjecthydrogen
dc.subjecthydrogen sulphide
dc.subjectmathematical modelling
dc.subjectmethane
dc.subjectmicrobiome
dc.titleCompetition for Hydrogen Prevents Coexistence of Human Gastrointestinal Hydrogenotrophs in Continuous Culture.
dc.typeJournal article
pubs.elements-id433077
pubs.organisational-groupOther
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