The effects of annual cycle, source population, and body condition on leukocyte profile and immune challenge in a basal reptile, the tuatara (Sphenodon punctatus).

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dc.citation.issue1
dc.citation.volume341
dc.contributor.authorLamar SK
dc.contributor.authorFrank HK
dc.contributor.authorLa Flamme A
dc.contributor.authorGartrell B
dc.contributor.authorOrmsby D
dc.contributor.authorNelson N
dc.coverage.spatialUnited States
dc.date.accessioned2024-08-05T02:36:38Z
dc.date.available2024-08-05T02:36:38Z
dc.date.issued2023-12-03
dc.description.abstractLeukocyte profiles are broadly used to assess the health status of many species. Reference intervals, and an understanding of the factors that may influence these intervals, are necessary for adequate interpretation of leukograms. Using a data set that spans over three decades, we investigated variation in leukocyte profile in several populations of the evolutionarily unique reptile, the tuatara (Sphenodon punctatus). To do this, we first established reference intervals for each leukocyte type according to best practices. Next, we determined that source population and sampling date were the two most important predictors of leukocyte makeup. We found significant differences in the ratio of heterophils: lymphocytes (H:L) between populations, with tuatara on the more resource-stressed sampling island having a significantly higher ratio of H:L. Finally, we found that sampling location, sex, and life stage did not explain variation in the responses of tuatara to stimulation with Concanavalin A and lipopolysaccharide in both 3-(4,5-dimethylthiazol-2-yl)-2,5-di-phenyltetrazolium bromide and Griess assay experiments. Our results offer important insight into the function of leukocytes in reptiles.
dc.description.confidentialfalse
dc.edition.editionJanuary 2024
dc.format.pagination60-72
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/37921244
dc.identifier.citationLamar SK, Frank HK, La Flamme A, Gartrell B, Ormsby D, Nelson N. (2024). The effects of annual cycle, source population, and body condition on leukocyte profile and immune challenge in a basal reptile, the tuatara (Sphenodon punctatus).. J Exp Zool A Ecol Integr Physiol. 341. 1. (pp. 60-72).
dc.identifier.doi10.1002/jez.2761
dc.identifier.eissn2471-5646
dc.identifier.elements-typejournal-article
dc.identifier.issn2471-5638
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71196
dc.languageeng
dc.publisherJohn Wiley and Sons, Inc.
dc.publisher.urihttps://onlinelibrary.wiley.com/doi/10.1002/jez.2761
dc.relation.isPartOfJ Exp Zool A Ecol Integr Physiol
dc.rights(c) 2023 The Author/s
dc.rightsCC BY-NC-ND 4.0
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.en
dc.subjectblood smears
dc.subjectimmunity
dc.subjectleukocytes
dc.subjectlymphocytes
dc.subjectreptiles
dc.subjectstress
dc.subjectAnimals
dc.subjectReptiles
dc.subjectLeukocytes
dc.titleThe effects of annual cycle, source population, and body condition on leukocyte profile and immune challenge in a basal reptile, the tuatara (Sphenodon punctatus).
dc.typeJournal article
pubs.elements-id484855
pubs.organisational-groupOther
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