Chitin Deacetylases Are Required for Epichloë festucae Endophytic Cell Wall Remodeling During Establishment of a Mutualistic Symbiotic Interaction with Lolium perenne

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dc.citation.issue10
dc.citation.volume34
dc.contributor.authorNoorifar N
dc.contributor.authorSavoian MS
dc.contributor.authorRam A
dc.contributor.authorLukito Y
dc.contributor.authorHassing B
dc.contributor.authorWeikert TW
dc.contributor.authorMoerschbacher BM
dc.contributor.authorScott B
dc.coverage.spatialUnited States
dc.date.accessioned2024-01-08T22:18:44Z
dc.date.accessioned2024-07-25T06:53:38Z
dc.date.available2021-10-28
dc.date.available2024-01-08T22:18:44Z
dc.date.available2024-07-25T06:53:38Z
dc.date.issued2021-10-28
dc.description.abstractEpichloë festucae forms a mutualistic symbiotic association with Lolium perenne. This biotrophic fungus systemically colonizes the intercellular spaces of aerial tissues to form an endophytic hyphal network and also grows as an epiphyte. However, little is known about the cell wall-remodeling mechanisms required to avoid host defense and maintain intercalary growth within the host. Here, we use a suite of molecular probes to show that the E. festucae cell wall is remodeled by conversion of chitin to chitosan during infection of L. perenne seedlings, as the hyphae switch from free-living to endophytic growth. When hyphae transition from endophytic to epiphytic growth, the cell wall is remodeled from predominantly chitosan to chitin. This conversion from chitin to chitosan is catalyzed by chitin deacetylase. The genome of E. festucae encodes three putative chitin deacetylases, two of which (cdaA and cdaB) are expressed in planta. Deletion of either of these genes results in disruption of fungal intercalary growth in the intercellular spaces of plants infected with these mutants. These results establish that these two genes are required for maintenance of the mutualistic symbiotic interaction between E. festucae and L. perenne.
dc.description.confidentialfalse
dc.edition.editionOctober 2021
dc.format.pagination1181-1192
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/34058838
dc.identifier.citationNoorifar N, Savoian MS, Ram A, Lukito Y, Hassing B, Weikert TW, Moerschbacher BM, Scott B. (2021). Chitin Deacetylases Are Required for Epichloë festucae Endophytic Cell Wall Remodeling During Establishment of a Mutualistic Symbiotic Interaction with Lolium perenne.. Mol Plant Microbe Interact. 34. 10. (pp. 1181-1192).
dc.identifier.doi10.1094/MPMI-12-20-0347-R
dc.identifier.eissn1943-7706
dc.identifier.elements-typejournal-article
dc.identifier.issn0894-0282
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71111
dc.languageeng
dc.publisherThe American Phytopathological Society in cooperation with the International Society for Molecular Plant-Microbe Interactions
dc.publisher.urihttps://apsjournals.apsnet.org/doi/10.1094/MPMI-12-20-0347-R
dc.relation.isPartOfMol Plant Microbe Interact
dc.rights(c) 2021 The Author/s
dc.rightsCC BY-NC-ND 4.0
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectEpichloëfestucae
dc.subjectchitin
dc.subjectchitosan
dc.subjectendophyte
dc.subjectmolecular probes
dc.subjectAmidohydrolases
dc.subjectCell Wall
dc.subjectChitin
dc.subjectEpichloe
dc.subjectFungal Proteins
dc.subjectGene Expression Regulation, Fungal
dc.subjectLolium
dc.subjectSymbiosis
dc.titleChitin Deacetylases Are Required for Epichloë festucae Endophytic Cell Wall Remodeling During Establishment of a Mutualistic Symbiotic Interaction with Lolium perenne
dc.typeJournal article
pubs.elements-id445924
pubs.organisational-groupOther
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