Virulence-associated genes in faecal and clinical Escherichia coli isolates cultured from broiler chickens in Australia

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dc.citation.volumeEarly View
dc.contributor.authorAwawdeh L
dc.contributor.authorForrest R
dc.contributor.authorTurni C
dc.contributor.authorCobbold R
dc.contributor.authorHenning J
dc.contributor.authorGibson J
dc.date.accessioned2024-07-10T01:55:09Z
dc.date.available2024-07-10T01:55:09Z
dc.date.issued2024-05-09
dc.description.abstractA healthy chicken's intestinal flora harbours a rich reservoir of Escherichia coli as part of the commensal microbiota. However, some strains, known as avian pathogenic E. coli (APEC), carry specific virulence genes (VGs) that enable them to invade and cause extraintestinal infections such as avian colibacillosis. Although several VG combinations have been identified, the pathogenic mechanisms associated with APEC are ill-defined. The current study screened a subset of 88 E. coli isolates selected from 237 pre-existing isolates obtained from commercial poultry flocks in Australia. The 88 isolates were selected based on their enterobacterial repetitive intergenic consensus (ERIC) and antimicrobial resistance (AMR) profiles and included 29 E. coli isolates cultured from chickens with colibacillosis (referred to as clinical E. coli or CEC) and 59 faecal E. coli (FEC) isolates cultured from clinically healthy chickens. The isolates were screened for the presence of 35 previously reported VGs. Of these, 34 were identified, with iucA not being detected. VGs focG, hlyA and sfa/foc were only detected in FEC isolates. Eight VGs had a prevalence of 90% or above in the CEC isolates. Specifically, astA (100%); feoB (96.6%); iutA, iss, ompT, iroN and hlyF (all 93.1%); and vat (89.7%). The prevalence of these were significantly lower in FEC isolates (astA 79.7%, feoB 77.9%, iutA 52.5%, iss 45.8%, ompT 50.9%, iroN 37.3%, hlyF 50.9% and vat 42.4%). The odds ratios that each of these eight VGs were more likely to be associated with CEC than FEC ranged from 7.8 to 21.9. These eight VGs may be used to better define APEC and diagnostically detect APEC in Australia. Further investigations are needed to identify the roles of these VGs in pathogenicity.
dc.description.confidentialfalse
dc.identifier.citationAwawdeh L, Forrest R, Turni C, Cobbold R, Henning J, Gibson J. (2024). Virulence-associated genes in faecal and clinical Escherichia coli isolates cultured from broiler chickens in Australia. Australian Veterinary Journal. Early View.
dc.identifier.doi10.1111/avj.13339
dc.identifier.eissn1751-0813
dc.identifier.elements-typejournal-article
dc.identifier.issn0005-0423
dc.identifier.numberavj.13339
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/70136
dc.languageEnglish
dc.publisherJohn Wiley and Sons Australia, Ltd on behalf of Australian Veterinary Association
dc.publisher.urihttps://onlinelibrary.wiley.com/doi/10.1111/avj.13339
dc.relation.isPartOfAustralian Veterinary Journal
dc.rights(c) 2024 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectantibiotic resistance proļ¬le
dc.subjectavian colibacillosis
dc.subjectavian pathogenic Escherichia coli
dc.subjectenterobacterial repetitive intergenic consensus
dc.subjectextraintestinal pathogenic E. coli
dc.subjectpolymerase chain reaction
dc.subjectvirulence genes
dc.titleVirulence-associated genes in faecal and clinical Escherichia coli isolates cultured from broiler chickens in Australia
dc.typeJournal article
pubs.elements-id489004
pubs.organisational-groupCollege of Health
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