Evaluation of single and multispecies biofilm formed in the static and continuous systems.

Pant K; Palmer J; Flint S

Evaluation of single and multispecies biofilm formed in the static and continuous systems.

dc.citation.volume	429
dc.contributor.author	Pant K
dc.contributor.author	Palmer J
dc.contributor.author	Flint S
dc.coverage.spatial	Netherlands
dc.date.accessioned	2025-01-08T02:13:27Z
dc.date.available	2025-01-08T02:13:27Z
dc.date.issued	2024-12-19
dc.description.abstract	Biofilms consisting of multiple species of bacteria compared to biofilms of single species are common in natural environments including food contact surfaces. The objective of this study was to understand the biofilm formation and the efficiency of sodium hypochlorite (50 ppm/5 mins) on the single and multiple species biofilm of Pseudomonas fluorescens, Staphylococcus aureus, and Listeria monocytogenes formed on stainless steel surfaces in static and continuous systems. The cell concentration of Listeria in the dual and triple species biofilm in the continuous system (7.3-8.4 log CFU/cm2) was higher compared to the static system (4.7-4.9 log CFU/cm2) while the concentration remained consistent in the single species biofilm (6.4-6.7 log CFU/cm2) for both systems. Biofilm formed in the static system was significantly (p < 0.001) more susceptible to sodium hypochlorite than biofilm formed in the continuous system. This observation agrees with the exopolysaccharide concentration which was found to be higher in the continuous system (8.0-15.6 μg/cm2) than in the static system (3.2-6.3 μg/cm2) indicating a positive correlation between EPS production and sanitizer resistance. Epifluorescence microscopy images showed the formation of interstitial voids within the three-species biofilm and filaments in the single and dual species Listeria biofilms in the continuous system which were absent in the static system. Overall, results showed that the biofilm formation and sanitizer resistance vary due to multispecies interaction and the presence of flow and should be considered an important variable in multispecies sanitizer resistance studies.
dc.description.confidential	false
dc.format.pagination	111030-
dc.identifier.author-url	https://www.ncbi.nlm.nih.gov/pubmed/39705888
dc.identifier.citation	Pant K, Palmer J, Flint S. (2024). Evaluation of single and multispecies biofilm formed in the static and continuous systems.. Int J Food Microbiol. 429. (pp. 111030-).
dc.identifier.doi	10.1016/j.ijfoodmicro.2024.111030
dc.identifier.eissn	1879-3460
dc.identifier.elements-type	journal-article
dc.identifier.issn	0168-1605
dc.identifier.number	111030
dc.identifier.pii	S0168-1605(24)00474-4
dc.identifier.uri	https://mro.massey.ac.nz/handle/10179/72335
dc.language	eng
dc.publisher	Elsevier B.V.
dc.publisher.uri	https://www.sciencedirect.com/science/article/pii/S0168160524004744
dc.relation.isPartOf	Int J Food Microbiol
dc.rights	(c) 2024 The Author/s
dc.rights	CC BY 4.0
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	Biofilm streamers
dc.subject	CDC bioreactor
dc.subject	Filaments
dc.subject	Flow channel
dc.subject	Sanitizer resistance
dc.subject	Stainless steel
dc.title	Evaluation of single and multispecies biofilm formed in the static and continuous systems.
dc.type	Journal article
pubs.elements-id	492799
pubs.organisational-group	Other