Mathematical modelling supports the existence of a threshold hydrogen concentration and media-dependent yields in the growth of a reductive acetogen.

Simple item page
dc.citation.issue5
dc.citation.volume43
dc.contributor.authorSmith NW
dc.contributor.authorShorten PR
dc.contributor.authorAltermann E
dc.contributor.authorRoy NC
dc.contributor.authorMcNabb WC
dc.coverage.spatialGermany
dc.date.accessioned2024-08-14T20:31:45Z
dc.date.available2024-08-14T20:31:45Z
dc.date.issued2020-05-01
dc.description.abstractThe bacterial production of acetate via reductive acetogenesis along the Wood-Ljungdahl metabolic pathway is an important source of this molecule in several environments, ranging from industrial bioreactors to the human gastrointestinal tract. Here, we contributed to the study of reductive acetogens by considering mathematical modelling techniques for the prediction of bacterial growth and acetate production. We found that the incorporation of a hydrogen uptake concentration threshold into the models improves their predictions and we calculated this threshold as 86.2 mM (95% confidence interval 6.1-132.6 mM). Monod kinetics and first-order kinetics models, with the inclusion of two candidate threshold terms or reversible Michaelis-Menten kinetics, were compared to experimental data and the optimal formulation for predicting both growth and metabolism was found. The models were then used to compare the efficacy of two growth media for acetogens. We found that the recently described general acetogen medium was superior to the DSMZ medium in terms of unbiased estimation of acetogen growth and investigated the contribution of yeast extract concentration to acetate production and bacterial growth in culture. The models and their predictions will be useful to those studying both industrially and environmentally relevant reductive acetogenesis and allow for straightforward adaptation to similar cases with different organisms.
dc.description.confidentialfalse
dc.edition.editionMay 2020
dc.format.pagination885-894
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/31982985
dc.identifier.citationSmith NW, Shorten PR, Altermann E, Roy NC, McNabb WC. (2020). Mathematical modelling supports the existence of a threshold hydrogen concentration and media-dependent yields in the growth of a reductive acetogen.. Bioprocess Biosyst Eng. 43. 5. (pp. 885-894).
dc.identifier.doi10.1007/s00449-020-02285-w
dc.identifier.eissn1615-7605
dc.identifier.elements-typejournal-article
dc.identifier.issn1615-7591
dc.identifier.pii10.1007/s00449-020-02285-w
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/71295
dc.languageeng
dc.publisherSpringer Nature Limited
dc.publisher.urihttps://link.springer.com/article/10.1007/s00449-020-02285-w
dc.relation.isPartOfBioprocess Biosyst Eng
dc.rights(c) 2022 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAcetate
dc.subjectHydrogen
dc.subjectMathematical modelling
dc.subjectSyngas
dc.subjectThreshold
dc.subjectYeast extract
dc.subjectClostridiales
dc.subjectCulture Media
dc.subjectHydrogen
dc.subjectModels, Biological
dc.titleMathematical modelling supports the existence of a threshold hydrogen concentration and media-dependent yields in the growth of a reductive acetogen.
dc.typeJournal article
pubs.elements-id429578
pubs.organisational-groupOther
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Published version.pdf
Size:
1.34 MB
Format:
Adobe Portable Document Format
Description:
429578 PDF.pdf
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
9.22 KB
Format:
Plain Text
Description:
Download
Collections
Journal Articles