Sandwich enzyme-linked immunosorbent assay (ELISA) analysis of plant cell wall glycan connections

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dc.contributor.authorCornuault VRG
dc.contributor.authorKnox JP
dc.date.available2014-04-20
dc.date.issued2014-04-20
dc.description.abstractSandwich ELISA is a highly sensitive method that can be used to determine if two epitopes are part of the same macromolecule or supramolecular complex. In the case of plant cell wall glycans, it can reveal the existence of inter-polymers linkages, leading to better understanding of overall cell wall architectures. This development of a conventional sandwich ELISA protocol uses a carbohydrate-binding module (CBM), a small protein domain found in some carbohydrate catalysing or activating enzymes, and rat monoclonal antibodies (mAbs) which can be combined in the same ELISA plate without risk of cross reaction; the secondary anti-rat HRP antibody being only able to bind to the rat mAb and not the CBM. This protocol was developed and modified in the Prof. J. Paul Knox lab at the University of Leeds
dc.identifier.citation2014
dc.identifier.doi10.21769/BioProtoc.1106
dc.identifier.elements-id403287
dc.identifier.harvestedMassey_Dark
dc.relation.urihttps://bio-protocol.org/e1106
dc.rights2014 The Authors; exclusive licensee Bio-protocol LLC.
dc.subjectELISA
dc.subjectPolysaccharide
dc.subjectCarbohydrate
dc.subjectPlant biochemistry
dc.titleSandwich enzyme-linked immunosorbent assay (ELISA) analysis of plant cell wall glycan connections
dc.typeinternet
pubs.notesNot known
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Sciences
pubs.organisational-group/Massey University/College of Sciences/Institute of Fundamental Sciences
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