Effects of Plantain (Plantago lanceolata L.) Metabolites Aucubin, Acteoside, and Catalpol on Methane Emissions In Vitro

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dc.citation.issue20
dc.citation.volume73
dc.contributor.authorSivanandarajah K
dc.contributor.authorDonaghy D
dc.contributor.authorKemp P
dc.contributor.authorNavarrete S
dc.contributor.authorHorne D
dc.contributor.authorRamilan T
dc.contributor.authorMolano G
dc.contributor.authorPacheco D
dc.date.accessioned2025-06-16T02:38:37Z
dc.date.available2025-06-16T02:38:37Z
dc.date.issued2025-05-21
dc.description.abstractPlantain (PL) contains plant secondary metabolites (PSM), such as acteoside, aucubin, and catalpol, known for their bioactive properties. While acteoside and aucubin have been linked to reducing nitrogen losses in grazed pastures, their effects on enteric methane (CH4) emissions remain unexplored. Three in vitro batch culture experiments were conducted to assess the effects of PSM on rumen fermentation, using PL pastures with varying PSM concentrations, purified PSM compounds, and/or their combinations added to ryegrass (Lolium perenne, RG), which does not contain these PSM. Aucubin addition to RG extended the time to reach halftime for gas production (GP) and CH4 by 15-20% due to its antimicrobial effects. Acteoside, alone or with aucubin, promoted propionate production, an alternative hydrogen sink, which reduced the acetate to propionate ratio, increased GP by up to 13%, and decreased CH4 proportion in gas by 5-15%. Aucubin reduced ruminal net ammonia (NH3) production by up to 46%, with a similar reduction observed when combined with acteoside. This study highlights the potential of PSM to mitigate CH4 emissions and reduce nitrogen losses from dairy cows, warranting in vivo evaluation of PSM and targeted breeding of PL pastures with increased PSM content.
dc.description.confidentialfalse
dc.format.pagination12132-12145
dc.identifier.citationSivanandarajah K, Donaghy D, Kemp P, Navarrete S, Horne D, Ramilan T, Molano G, Pacheco D. (2025). Effects of Plantain (Plantago lanceolata L.) Metabolites Aucubin, Acteoside, and Catalpol on Methane Emissions In Vitro. Journal of Agricultural and Food Chemistry. 73. 20. (pp. 12132-12145).
dc.identifier.doi10.1021/acs.jafc.4c12140
dc.identifier.eissn1520-5118
dc.identifier.elements-typejournal-article
dc.identifier.issn0021-8561
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/73058
dc.languageEnglish
dc.publisherAmerican Chemical Society
dc.publisher.urihttps://pubs.acs.org/doi/10.1021/acs.jafc.4c12140
dc.relation.isPartOfJournal of Agricultural and Food Chemistry
dc.rights(c) 2025 The Author/s
dc.rightsCC BY-NC-ND 4.0
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectbioactive compounds
dc.subjectverbascoside
dc.subjectdairy
dc.subjectpasture
dc.subjectrumen fluid
dc.subjectammonia
dc.subjectnitrogen losses
dc.titleEffects of Plantain (Plantago lanceolata L.) Metabolites Aucubin, Acteoside, and Catalpol on Methane Emissions In Vitro
dc.typeJournal article
pubs.elements-id500877
pubs.organisational-groupOther
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