Multi-scale spatial heterogeneity of pectic rhamnogalacturonan I (RG-I) structural features in tobacco seed endosperm cell walls

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dc.citation.issue6
dc.citation.volume75
dc.contributor.authorLee KJD
dc.contributor.authorCornuault VRG
dc.contributor.authorManfield IW
dc.contributor.authorRalet MC
dc.contributor.authorKnox JP
dc.date.available2013-09-03
dc.date.issued2013-09-03
dc.description.abstractPlant cell walls are complex configurations of polysaccharides that fulfil a diversity of roles during plant growth and development. They also provide sets of biomaterials that are widely exploited in food, fibre and fuel applications. The pectic polysaccharides, which comprise approximately a third of primary cell walls, form complex supramolecular structures with distinct glycan domains. Rhamnogalacturonan I (RG-I) is a highly structurally heterogeneous branched glycan domain within the pectic supramolecule that contains rhamnogalacturonan, arabinan and galactan as structural elements. Heterogeneous RG-I polymers are implicated in generating the mechanical properties of cell walls during cell development and plant growth, but are poorly understood in architectural, biochemical and functional terms. Using specific monoclonal antibodies to the three major RG-I structural elements (arabinan, galactan and the rhamnogalacturonan backbone) for in situ analyses and chromatographic detection analyses, the relative occurrences of RG-I structures were studied within a single tissue: the tobacco seed endosperm. The analyses indicate that the features of the RG-I polymer display spatial heterogeneity at the level of the tissue and the level of single cell walls, and also heterogeneity at the biochemical level. This work has implications for understanding RG-I glycan complexity in the context of cell-wall architectures and in relation to cell-wall functions in cell and tissue development.
dc.description.confidentialfalse
dc.format.extent1018 - 1027
dc.identifier.citationThe Plant Journal, 2013, 75 (6), pp. 1018 - 1027
dc.identifier.doi10.1111/tpj.12263
dc.identifier.elements-id403213
dc.identifier.harvestedMassey_Dark
dc.identifier.issn0960-7412
dc.languageenglish
dc.publisherWiley
dc.relation.isPartOfThe Plant Journal
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3824205/#__ffn_sectitle
dc.rights© 2013 John Wiley & Sons Ltd and the Society for Experimental Biology Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation
dc.subjectNicotiana tabacum
dc.subjectcell wall
dc.subjectpectin
dc.subjectrhamnogalacturonan-I
dc.subjectseed endosperm
dc.subject.anzsrc0601 Biochemistry and Cell Biology
dc.subject.anzsrc0607 Plant Biology
dc.titleMulti-scale spatial heterogeneity of pectic rhamnogalacturonan I (RG-I) structural features in tobacco seed endosperm cell walls
dc.typeJournal article
pubs.notesNot known
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Sciences
pubs.organisational-group/Massey University/College of Sciences/Institute of Fundamental Sciences
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