Identification of Genomic Regions Associated with Concentrations of Milk Fat, Protein, Urea and Efficiency of Crude Protein Utilization in Grazing Dairy Cows

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dc.citation.issue3
dc.citation.volume12
dc.contributor.authorAriyarathne HBPC
dc.contributor.authorCorrea-Luna M
dc.contributor.authorBlair HT
dc.contributor.authorGarrick DJ
dc.contributor.authorLopez-Villalobos N
dc.coverage.spatialSwitzerland
dc.date.accessioned2024-01-10T01:32:57Z
dc.date.accessioned2024-07-25T06:35:31Z
dc.date.available2021-03-23
dc.date.available2024-01-10T01:32:57Z
dc.date.available2024-07-25T06:35:31Z
dc.date.issued2021-03-23
dc.description.abstractAbstract The objective of this study was to identify genomic regions associated with milk fat percentage (FP), crude protein percentage (CPP), urea concentration (MU) and efficiency of crude protein utilization (ECPU: ratio between crude protein yield in milk and dietary crude protein intake) using grazing, mixed-breed, dairy cows in New Zealand. Phenotypes from 634 Holstein Friesian, Jersey or crossbred cows were obtained from two herds at Massey University. A subset of 490 of these cows was genotyped using Bovine Illumina 50K SNP-chips. Two genome-wise association approaches were used, a single-locus model fitted to data from 490 cows and a single-step Bayes C model fitted to data from all 634 cows. The single-locus analysis was performed with the Efficient Mixed-Model Association eXpedited model as implemented in the SVS package. Single nucleotide polymorphisms (SNPs) with genome-wide association p-values ≤ 1.11 × 10−6 were considered as putative quantitative trait loci (QTL). The Bayes C analysis was performed with the JWAS package and 1-Mb genomic windows containing SNPs that explained > 0.37% of the genetic variance were considered as putative QTL. Candidate genes within 100 kb from the identified SNPs in single-locus GWAS or the 1-Mb windows were identified using gene ontology, as implemented in the Ensembl Genome Browser. The genes detected in association with FP (MGST1, DGAT1, CEBPD, SLC52A2, GPAT4, and ACOX3) and CPP (DGAT1, CSN1S1, GOSR2, HERC6, and IGF1R) were identified as candidates. Gene ontology revealed six novel candidate genes (GMDS, E2F7, SIAH1, SLC24A4, LGMN, and ASS1) significantly associated with MU whose functions were in protein catabolism, urea cycle, ion transportation and N excretion. One novel candidate gene was identified in association with ECPU (MAP3K1) that is involved in post-transcriptional modification of proteins. The findings should be validated using a larger population of New Zealand grazing dairy cows.
dc.format.pagination456-
dc.identifier.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/33806889
dc.identifier.citationAriyarathne HBPC, Correa-Luna M, Blair HT, Garrick DJ, Lopez-Villalobos N. (2021). Identification of Genomic Regions Associated with Concentrations of Milk Fat, Protein, Urea and Efficiency of Crude Protein Utilization in Grazing Dairy Cows.. Genes (Basel). 12. 3. (pp. 456-).
dc.identifier.doi10.3390/genes12030456
dc.identifier.eissn2073-4425
dc.identifier.elements-typejournal-article
dc.identifier.issn2073-4425
dc.identifier.numberARTN 456
dc.identifier.piigenes12030456
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/70500
dc.languageeng
dc.publisherMDPI (Basel, Switzerland)
dc.relation.isPartOfGenes (Basel)
dc.rights(c) 2021 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectcandidate gene
dc.subjectdairy cows
dc.subjectefficiency of crude protein utilization
dc.subjectgenome-wide association studies
dc.subjectmilk urea concentration
dc.subjectsingle nucleotide polymorphism
dc.subjectAnimals
dc.subjectBreeding
dc.subjectCattle
dc.subjectFatty Acids
dc.subjectFemale
dc.subjectGenome-Wide Association Study
dc.subjectHerbivory
dc.subjectMilk
dc.subjectMilk Proteins
dc.subjectNew Zealand
dc.subjectPolymorphism, Single Nucleotide
dc.subjectQuantitative Trait Loci
dc.subjectUrea
dc.titleIdentification of Genomic Regions Associated with Concentrations of Milk Fat, Protein, Urea and Efficiency of Crude Protein Utilization in Grazing Dairy Cows
dc.typeJournal article
pubs.elements-id443093
pubs.organisational-groupOther
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