The LONELY GUY gene family: from mosses to wheat, the key to the formation of active cytokinins in plants

Chen L; Jameson GB; Guo Y; Song J; Jameson PE

The LONELY GUY gene family: from mosses to wheat, the key to the formation of active cytokinins in plants

dc.citation.issue	4
dc.citation.volume	20
dc.contributor.author	Chen L
dc.contributor.author	Jameson GB
dc.contributor.author	Guo Y
dc.contributor.author	Song J
dc.contributor.author	Jameson PE
dc.coverage.spatial	England
dc.date.accessioned	2023-12-15T01:28:04Z
dc.date.accessioned	2024-07-25T06:34:33Z
dc.date.available	2022-03
dc.date.available	2023-12-15T01:28:04Z
dc.date.available	2024-07-25T06:34:33Z
dc.date.issued	2022-04-07
dc.description.abstract	LONELY GUY (LOG) was first identified in a screen of rice mutants with defects in meristem maintenance. In plants, LOG codes for cytokinin riboside 5'-monophosphate phosphoribohydrolase, which converts inactive cytokinin nucleotides directly to the active free bases. Many enzymes with the PGGxGTxxE motif have been misannotated as lysine decarboxylases; conversely not all enzymes containing this motif are cytokinin-specific LOGs. As LOG mutants clearly impact yield in rice, we investigated the LOG gene family in bread wheat. By interrogating the wheat (Triticum aestivum) genome database, we show that wheat has multiple LOGs. The close alignment of TaLOG1, TaLOG2 and TaLOG6 with the X-ray structures of two functional Arabidopsis thaliana LOGs allows us to infer that the wheat LOGs 1-11 are functional LOGs. Using RNA-seq data sets, we assessed TaLOG expression across 70 tissue types, their responses to various stressors, the pattern of cis-regulatory elements (CREs) and intron/exon patterns. TaLOG gene family members are expressed variously across tissue types. When the TaLOG CREs are compared with those of the cytokinin dehydrogenases (CKX) and glucosyltransferases (CGT), there is close alignment of CREs between TaLOGs and TaCKXs reflecting the key role of CKX in maintaining cytokinin homeostasis. However, we suggest that the main homeostatic mechanism controlling cytokinin levels in response to biotic and abiotic challenge resides in the CGTs, rather than LOG or CKX. However, LOG transgenics and identified mutants in rice variously impact yield, providing interesting avenues for investigation in wheat.
dc.description.confidential	false
dc.edition.edition	April 2022
dc.format.pagination	625-645
dc.identifier.author-url	https://www.ncbi.nlm.nih.gov/pubmed/35108444
dc.identifier.citation	Chen L, Jameson GB, Guo Y, Song J, Jameson PE. (2022). The LONELY GUY gene family: from mosses to wheat, the key to the formation of active cytokinins in plants.. Plant Biotechnol J. 20. 4. (pp. 625-645).
dc.identifier.doi	10.1111/pbi.13783
dc.identifier.eissn	1467-7652
dc.identifier.elements-type	journal-article
dc.identifier.uri	https://mro.massey.ac.nz/handle/10179/70447
dc.language	eng
dc.publisher	John Wiley and Sons Ltd on behalf of the Society for Experimental Biology and The Association of Applied Biologists
dc.publisher.uri	https://onlinelibrary.wiley.com/doi/10.1111/pbi.13783
dc.relation.isPartOf	Plant Biotechnol J
dc.rights	(c) 2022 The Author/s
dc.rights	CC BY-NC 4.0
dc.rights.uri	https://creativecommons.org/licenses/by-nc/4.0/
dc.subject	5′-monophosphate phosphoribohydrolase
dc.subject	LOG
dc.subject	LONELY GUY
dc.subject	cis-regulatory elements
dc.subject	cytokinin
dc.subject	cytokinin riboside
dc.subject	wheat
dc.subject	yield
dc.subject	Arabidopsis
dc.subject	Bryophyta
dc.subject	Cytokinins
dc.subject	Gene Expression Regulation, Plant
dc.subject	Oryza
dc.subject	Triticum
dc.title	The LONELY GUY gene family: from mosses to wheat, the key to the formation of active cytokinins in plants
dc.type	Journal article
pubs.elements-id	451077
pubs.organisational-group	Other