Proteins isolated with TRIzol are compatible with two-dimensional electrophoresis and mass spectrometry analyses

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dc.citation.issue1
dc.citation.volume421
dc.contributor.authorYoung C
dc.contributor.authorTruman P
dc.date.available2012
dc.date.issued2012
dc.description.abstractTRIzol is used for RNA isolation but also permits protein recovery. We investigated whether proteins prepared with TRIzol were suitable for two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization mass spectrometry. Proteins from TRIzol-treated SH-SY5Y cells produced 2-DE spot patterns similar to those from an equivalent untreated sample. Subsequent identification of TRIzol-treated proteins using peptide mass fingerprinting was successful. TRIzol exposure altered neither the mass of myoglobin extracted from sodium dodecyl sulfate (SDS) gels nor the masses of myoglobin peptides produced by in-gel trypsin digestion. These findings suggest that proteins isolated with TRIzol remain amenable to proteomic analyses.
dc.description.confidentialfalse
dc.description.notesCited By :5 Export Date: 28 January 2015
dc.format.extent330 - 332
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84855893383&partnerID=40&md5=92683035ddff26d68db9c41633195bea
dc.identifier.citationAnalytical Biochemistry, 2012, 421 (1), pp. 330 - 332
dc.identifier.doi10.1016/j.ab.2011.10.045
dc.identifier.elements-id220813
dc.identifier.issn1096-0309
dc.publisherElsevier Masson
dc.relation.isPartOfAnalytical Biochemistry
dc.subject2-DE
dc.subjectMass spectrometry
dc.subjectPeptide mass fingerprint
dc.subjectProtein extraction
dc.subjectProteomics
dc.subjectTRIzol
dc.subject.anzsrc0301 Analytical Chemistry
dc.subject.anzsrc0399 Other Chemical Sciences
dc.subject.anzsrc0601 Biochemistry and Cell Biology
dc.titleProteins isolated with TRIzol are compatible with two-dimensional electrophoresis and mass spectrometry analyses
dc.typeJournal article
pubs.notesNot known
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Health
pubs.organisational-group/Massey University/College of Health/School of Health Science
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