A simple screen to identify promoters conferring high levels of phenotypic noise.

dc.citation.issue12
dc.citation.volume4
dc.contributor.authorFreed NE
dc.contributor.authorSilander OK
dc.contributor.authorStecher B
dc.contributor.authorBöhm A
dc.contributor.authorHardt W-D
dc.contributor.authorAckermann M
dc.date.available2008-12-01
dc.date.available2008-11-13
dc.date.issued2008-12
dc.descriptionCreative Commons Attribution License
dc.description.abstractGenetically identical populations of unicellular organisms often show marked variation in some phenotypic traits. To investigate the molecular causes and possible biological functions of this phenotypic noise, it would be useful to have a method to identify genes whose expression varies stochastically on a certain time scale. Here, we developed such a method and used it for identifying genes with high levels of phenotypic noise in Salmonella enterica ssp. I serovar Typhimurium (S. Typhimurium). We created a genomic plasmid library fused to a green fluorescent protein (GFP) reporter and subjected replicate populations harboring this library to fluctuating selection for GFP expression using fluorescent-activated cell sorting (FACS). After seven rounds of fluctuating selection, the populations were strongly enriched for promoters that showed a high amount of noise in gene expression. Our results indicate that the activity of some promoters of S. Typhimurium varies on such a short time scale that these promoters can absorb rapid fluctuations in the direction of selection, as imposed during our experiment. The genomic fragments that conferred the highest levels of phenotypic variation were promoters controlling the synthesis of flagella, which are associated with virulence and host-pathogen interactions. This confirms earlier reports that phenotypic noise may play a role in pathogenesis and indicates that these promoters have among the highest levels of noise in the S. Typhimurium genome. This approach can be applied to many other bacterial and eukaryotic systems as a simple method for identifying genes with noisy expression.
dc.description.publication-statusPublished
dc.format.extent? - ? (6)
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000263667900022&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=c5bb3b2499afac691c2e3c1a83ef6fef
dc.identifierARTN e1000307
dc.identifier.citationPLOS GENETICS, 2008, 4 (12), pp. ? - ? (6)
dc.identifier.doi10.1371/journal.pgen.1000307
dc.identifier.elements-id235589
dc.identifier.harvestedMassey_Dark
dc.identifier.issn1553-7390
dc.languageEnglish
dc.publisherPUBLIC LIBRARY SCIENCE
dc.relation.isPartOfPLOS GENETICS
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectGenetics & Heredity
dc.subjectGENETICS & HEREDITY
dc.subjectENTERICA SEROVAR TYPHIMURIUM
dc.subjectTOLL-LIKE RECEPTOR-5
dc.subjectGENE-EXPRESSION
dc.subjectSALMONELLA-ENTERICA
dc.subjectSINGLE-CELL
dc.subjectPHASE VARIATION
dc.subjectFLAGELLIN
dc.subjectMICE
dc.subjectMACROPHAGES
dc.subjectDIVERSITY
dc.subject.anzsrc0604 Genetics
dc.titleA simple screen to identify promoters conferring high levels of phenotypic noise.
dc.typeJournal article
pubs.notesNot known
pubs.organisational-group/Massey University
pubs.organisational-group/Massey University/College of Sciences
pubs.organisational-group/Massey University/College of Sciences/School of Natural and Computational Sciences
pubs.organisational-group/Massey University/College of Sciences/School of Natural Sciences
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